郑华,莫妮妹,戴妍,林捷,李航宇,郑夏彤,吴伟彬.鸡肉酶解物提高小鼠的抗疲劳及抗氧化活性[J].,2020,36(6):9-16.
鸡肉酶解物提高小鼠的抗疲劳及抗氧化活性
Chicken Enzymatic Hydrolysates Improved Anti-fatigue and Antioxidant Activities in Mice
投稿时间:2019-11-06  
DOI:10.13982/j.mfst.1673-9078.2020.6.1079
中文关键词:  鸡肉酶解物  抗疲劳  抗氧化活性
英文关键词:chicken enzymatic hydrolysates  anti-fatigue  antioxidant enzyme activity
作者简介:郑华(1966-),男,副教授,硕士,研究方向:畜禽产品加工
基金项目:国家肉鸡产业体系(CARS-41-G16);广东省畜禽肉类农产品精深加工项目[粤财农(2017)100号];广东省家禽产业技术体系创新团队建设项目(2019KJ128);广东省大学生创新创业训练计划项目(201810564121)
作者单位
郑华 (1.华南农业大学食品学院,广东广州 510642) 
莫妮妹 (1.华南农业大学食品学院,广东广州 510642) 
戴妍 (1.华南农业大学食品学院,广东广州 510642) 
林捷 (1.华南农业大学食品学院,广东广州 510642)(2.畜禽产品精准加工和安全控制技术国家地方联合工程研究中心,广东广州 510642) 
李航宇 (1.华南农业大学食品学院,广东广州 510642) 
郑夏彤 (1.华南农业大学食品学院,广东广州 510642) 
吴伟彬 (1.华南农业大学食品学院,广东广州 510642) 
AuthorInstitution
ZHENG Hua (1.College of Food Science, South China Agricultural University, Guangzhou 510642, China) 
MO Ni-mei (1.College of Food Science, South China Agricultural University, Guangzhou 510642, China) 
DAI Yan (1.College of Food Science, South China Agricultural University, Guangzhou 510642, China) 
LIN Jie (1.College of Food Science, South China Agricultural University, Guangzhou 510642, China) (2.National and Local Joint Engineering Research Center of Precision Processing and Safety Control Technology for Livestock and Poultry Products, Guangzhou 510642, China) 
LI Hang-yu (1.College of Food Science, South China Agricultural University, Guangzhou 510642, China) 
ZHENG Xia-tong (1.College of Food Science, South China Agricultural University, Guangzhou 510642, China) 
WU Wei-bin (1.College of Food Science, South China Agricultural University, Guangzhou 510642, China) 
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中文摘要:
      论文研究了鸡肉酶解物EP1和EP2的抗疲劳及抗氧化效果。以生理盐水及鸡肉匀浆[蛋白质剂量200 mg/(kg?bw?d)]作为对照组,以40 mg/(kg?bw?d)和200 mg/(kg?bw?d)两个剂量的鸡肉酶解物EP1和EP2对实验小鼠进行灌胃实验28 d后,测定小鼠的力竭游泳时间、游泳30 min后的血清尿素氮含量、血清乳酸含量及肝糖原含量,并分析了小鼠血清中SOD(超氧化物歧化酶)、GSH-Px.(谷胱甘肽过氧化物酶)和CAT(过氧化氢酶)等主要抗氧化酶的活性。结果显示,鸡肉酶解物能够有效延长小鼠的力竭游泳时间(SC组为766 s,而H-EP1组可达到2307 s);显著降低游泳30 min后小鼠的血清乳酸含量(SC组为10.06 mmol/L,H-EP1组和H-EP2均为6.66 mmol/L)及血清尿素氮含量(SC组为19.72 mmol/L,H-EP1组仅为13.34 mmol/L),提高肝糖原含量(SC组为5.11 mg/g,H-EP2组可达到8.35 mg/g);显著提高游泳30 min后小鼠的血清中SOD(从89.08 U/mL提高到160.13 U/mL)、CAT(从0.25 U/mL U/mL提高到0.29 U/mL)和GSH-Px.(从257.36 U/mL提高到597.08 U/mL)等抗氧化酶的活性。研究结果提示,鸡肉酶解物EP1和EP2能够显著延长实验小鼠的力竭游泳时间,减少实验小鼠激烈运动时肝糖原的消耗和乳酸的产生与沉积,促进实验小鼠体内蛋白质的代谢及有效清除血清尿素氮,提高实验小鼠机体的血清SOD、CAT及GSH-Px.等抗氧化酶的活性,增强机体清除自由基的能力,改善小鼠的运动状况,确保小鼠在激烈运动状态下机体处于有氧运动模式,从而降低激烈运动后机体的疲劳程度,具有显著的抗疲劳和抗氧化效果。
英文摘要:
      This study investigated the anti-fatigue and anti-oxidation effects of chicken enzymatic hydrolysates EP1 and EP2. Normal saline and chicken homogenate [protein dose 200 mg/(kg?bw?d)] were used as the control group, and two doses of EP1 and EP2, 40 mg/(kg?bw?d) and 200 mg/(kg?bw?d), were administered by gavage into the mice. After 28 days of administration, the exhausted swimming time, and the serum urea nitrogen content, serum lactic acid content and liver glycogen content in the mice after 30 min of swimming were measured. The activities of SOD (superoxide dismutase), GSH-Px. (glutathione peroxidase) and CAT (catalase) in mouse serum were also analyzed. The results showed that chicken hydrolysates could effectively prolong the exhausted swimming time of mice (the SC group 766 s; the H-EP1 group 2307 s). After 30 min of swimming, the contents of serum lactic acid (the SC group 10.06 mmol/L; both the H-EP1 and H-EP2 groups 6.66 mmol/L) and serum urea nitrogen (the SC group 19.72 mmol/L; the H-EP1 group 13.34 mmol/L) decreased significantly, whilst the content of liver glycogen (the SC group 5.11 mg/g; the H-EP2 group 8.35 mg/g) as well as the activities of serum SOD, GSH-Px. and CAT antioxidant enzymes increased significantly. The research results suggest that chicken enzymatic hydrolysates EP1 and EP2 could significantly extend the exhaustion swimming time of mice, reduced the consumption of liver glycogen and the production and deposition of lactic acid in mice after intense exercise, promoted protein metabolism, removed effectively the serum urea nitrogen in mice, increased the antioxidant activities of serum SOD (from 89.08 to160.13 U/mL), CAT (from 0.25 to 0.29 U/mL) and GSH-Px. (from 257.36 to 597.08 U/mL) in mice, enhance the free radical scavenging ability of mice, and improved the exercise status of mice. These changes ensure that the mice subjected to intense exercise remained in the mode of aerobic exercise, so as to reduce the body’s fatigue after intense exercise, with significant anti-fatigue and anti-oxidation effects.
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