研究市售猪肉源金黄色葡萄球菌的污染情况，测定季铵盐类消毒剂对分离菌株MIC范围，以及耐消毒剂基因携带情况。采集春夏季与秋冬季市售生猪肉样品576份，采用肉汤稀释法测定苯扎溴铵对分离菌株的MIC，采用PCR技术对分离菌株5种耐消毒剂相关基因（qacA/B、qacC/D、qacG、qacH、qacJ）进行检测。576份样品中分离得到297株金黄色葡萄球菌，总分离率为51.56%；其中，春夏季样品374份，分离出158株；秋冬季样品202份，分离出139株。苯扎溴铵对分离菌株的MIC在0.00125~0.01 μg/mL，297株中168株检出携带耐消毒剂基因，总检出率为56.6%，qacA/B、qacC/D、qacG、qacH、qacJ的检出率分别为2.36%、19.86%、40.74%、6.73%、0.67%。结果表明猪肉源金黄色葡萄球菌的分离率和菌株消毒剂抗性基因携带率较高，猪肉中分离的金黄色葡萄球菌对季铵盐类消毒剂敏感。本研究为市售猪肉金黄色葡萄球菌的防控及消除提供参考。

In this work, the pollution of Staphylococcus aureus from pork purchased from the market was investigated. The MIC ranges of quaternary ammonium disinfectant to the isolated strains and the disinfectant resistant genes carrying were detected. The 576 samples of the commercial raw pork in four seasons were collected. The MIC values of the isolated strains were determined by broth dilution methods. The five anti-disinfectant-related genes (qacA/ B, qacC/ D, qacG, qacH, qacJ) were detected by PCR. The 297 strains of S. aureus were isolated from 576 pork source samples, and the total isolation rates was 51.56%. The 158 S. aureus strains were isolated from 374 samples that collected in spring and summer. The 139 S. aureus strains were isolated from 202 samples that collected in autumn and winter. The MIC ranges to benzalkonium bromide were 0.00125~0.01 μg/mL. Among 297 strains of S. aureus, 168 strains were found to carry the disinfectant-resistant genes, and the total detection rate was 56.6%. The positive detection rates for qacA/B, qacC/D, qacG, qacH, and qacJ were 2.36%, 19.86%, 40.74%, 6.73%, 0.67%, respectively. The result showed that the isolation rates from pork source and the resistance genes to disinfectant were relatively high. The S. aureus isolated from pork were sensitive to the quaternary salt-based disinfectant. This study will provides reference for the prevention and control of S. aureus pork isolates.

国家重点研发计划项目(2017YFC1600100)；四川省科技计划项目（2018JY0540，2019YJ0261)；西南民族大学研究生创新项目（CX2019SZ156）