[关键词]
[摘要]
观察复方铁皮石斛叶超微粉不同配比对高脂高糖致高脂血症模型小鼠的影响。采用高脂高糖饲料喂养制备高脂血症模型小鼠,造模成功后灌胃给予复方铁皮石斛叶超微粉(铁皮石斛叶超微粉和山楂精提物不同配比依次为1:1、1:2、1:4、2:1、4:1)连续8周。检测血清中胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-c)、低密度脂蛋白胆固醇(LDL-c)、谷草转氨酶(ALT)和谷丙转氨酶(AST)水平;末次给药后,检测血清超氧化物歧化酶(SOD)、丙二醛(MDA)和氧化低密度脂蛋白(Ox-LDL)水平;检测肝脏中胆固醇(TC);HE染色观察小鼠肝脏组织形态学。结果表明,复方铁皮石斛叶超微粉(4:1)能有效缓解高脂血症,降低血清TC、LDL-c、MDA、Ox-LDL水平,下降幅度分别为40.84%、36.67%、5.69%、10.40%(p<0.01),血清SOD活性提高5.47%(p<0.01);肝脏TC水平下降14.10%(p<0.05);肝脏重量降低31.35%(p<0.01),能改善肝脏中脂肪沉积。上述结果明确了复方铁皮石斛叶超微粉(4:1)给药组效果较好,可以改善高脂高糖致小鼠血脂异常、肝脏病变。
[Key word]
[Abstract]
To observe the effects of different proportions of compound Dendrobium officinale leaf ultrafine powder on the hyperlipidemia model mice induced by a high-fat and high-sugar diet. After successful modeling, the mice were administered with the compound Dendrobium officinale leaf ultrafine powder (with the ratio of the Dendrobium candidum leaf superfine powder to the refined extract of hawthorn as 1:1, 1:2, 1:4, 2:1, 4:1) for 8 weeks. The serum levels of total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-c), low density lipoprotein cholesterol (LDL-c), glutamic-oxaloacetic transaminase (ALT), and alanine-glutamic transaminase (AST) were measured. After the last administration, the serum levels of superoxide dismutase (SOD), malondialdehyde (MDA) and oxidized low density lipoprotein (Ox-LDL) were measured. The cholesterol level in the liver was measured, and the liver tissue of the mice was examined by HE staining. The results showed that the compound Dendrobium officinale leaf ultrafine powder (at a ratio of 4:1) could effectively alleviate hyperlipidemia: decreased the serum levels of TC, LDL-c, MDA and Ox-LDL by 40.84%, 36.67%, 5.05% and 10.40% respectively (p<0.01), increased the serum SOD activity by 5.47% (p<0.01), decreased the liver TC level by 14.10% (p<0.01) and liver weight by 31.35% (p<0.01), and ameliorated liver fat deposition. The above results confirmed that the compound Dendrobium officinale leaf ultrafine powder (4:1) was effective and could ameliorate the dyslipidemia and liver lesions in mice induced by a high-fat and high-sugar diet.
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[基金项目]
国家重点研发计划项目(2017YFC170203);国家自然科学基金项目(81703772)