本文利用非糖物质快速鉴别四种常见的单花蜂蜜（枸杞蜂蜜、荆条蜂蜜、枣花蜂蜜和洋槐蜂蜜）。利用固相萃取除糖提取蜂蜜非糖物质，并用高效液相色谱（HPLC）结合化学计量学进行蜂蜜花源的判定。发现了四种单花蜂蜜的非糖特征标志物。芦丁、丁香酸甲酯、阿魏酸的含量在四种蜂蜜中差异最大。对羟基苯甲酸和脱落酸在枸杞蜂蜜中平均含量分别为0.59 μg/g和0.42 μg/g，可作为枸杞蜂蜜的花源标志物；对香豆酸和肉桂酸在荆条蜂蜜中平均含量为0.08 μg/g和0.05 μg/g，可作为荆条蜂蜜的潜在花源标志物；阿魏酸、异阿魏酸是枣花蜂蜜的特征标志物，平均含量分别为0.40 μg/g和0.76 μg/g；洋槐蜂蜜的特征标志物是芦丁和丁香酸甲酯，平均含量为0.08 μg/g和0.14 μg/g。利用高效液相色谱指纹图谱结合化学计量学方法（主成分分析和判别分析），四种单花蜂蜜依据其花源被成功区分。本研究对蜂蜜真实性判定有重要参考价值，为后续蜂蜜真伪鉴别奠定基础。

In order to quickly identify four common monofloral honeys (Medlar honey, Chaste honey, Jujube honey and Locust honey), non-sugar substances were used in this work. The non-sugar substances of honey were extracted by solid phase extraction (SPE) and sugar was removed. The determination of honey floral origin was carried out by high performance liquid chromatography (HPLC) combined with chemometrics. The non-sugar characteristic markers of these four monofloral honeys were found. The contents of rutin, methyl syringate and ferulic acid were significantly different among the four monofloral honeys. P-hydroxybenzoic acid and abscisic acid can be considered as floral origin markers of medlar honey with an average content of 0.59 μg/g and 0.42 μg/g, respectively. The average content of p-coumaric and cinnamic acid in chaste honey is 0.08 μg/g and 0.05 μg/g, respectively, which can be used as potential flower origin markers for chaste honey. Ferulic acid and isoferulic acid can be the characteristic markers of jujube honey with an average content of 0.40 μg/g and 0.76 μg/g, respectively. The characteristic markers of locust honey are rutin and methyl syringate with an average content of 0.08 μg/g and 0.14 μg/g, respectively. HPLC fingerprint combined with chemometrics (principal component analysis and discriminant analysis) was used, and the four monofloral honeys were successfully distinguished according to their floral origins. This study has important reference value for the determination of honey authenticity, which lays a foundation for the subsequent identification of honey.

国家重点研发计划项目(2016YFD0400802)