[关键词]
[摘要]
以三文鱼骨为原料制备三文鱼骨胶原低聚肽,与钙离子螯合为三文鱼骨胶原低聚肽钙并作为受试物,以低中高剂量作用SD大鼠,并设置空白组和碳酸钙阳性对照组,检测体重、钙吸收率与储留率、股骨干重、股骨长、骨密度等重要指标,评价三文鱼骨胶原低聚肽钙的增加骨密度功能。结果显示,螯合物的总蛋白含量为65.37%,螯合率为52.56%,螯合物得率为43.12%;各试验组体重无显著性差异(p>0.05),中、高剂量组和碳酸钙组的钙吸收率分别为39.18%、40.52%、38.38%,储留率分别为38.80%、39.27%、37.58%,显著高于空白对照组36.31%,35.91%(p<0.05),且高剂量组明显优于同等钙含量的碳酸钙组(p<0.05);另外高剂量组的股骨干重、股骨中点直径分别为0.57(g)和2.40(mm),股骨近端、远端和中点密度分别为0.19、0.22、0.17(g/cm2),均高于空白组(p<0.05),与碳酸钙组无显著性差异。表明三文鱼骨胶原低聚肽钙具有良好的促钙吸收效果和增加骨密度的功能,为相关新型功能性食品的开发利用奠定良好的理论基础。
[Key word]
[Abstract]
Salmon bone collagen oligopeptide was prepared from salmon bone and chelated with calcium ion as salmon collagen oligopeptide calcium and used as test substance. Sprague-Dawley rats were orally administered with low, medium and high doses of test substances, and a blank control group and a calcium carbonate positive control group were used. The body weight, calcium absorption rate and retention rate, femur dry weight, femur length, bone density and other important indicators were measured to evaluate the bone mineral density of the salmon collagen oligopeptide calcium. The results showed that the total protein content of salmon collagen oligopeptide calcium was 65.37%, the chelation rate was 52.56%, and the chelate yield was 43.12±0.13%. There was no significant difference in body weight between the experimental groups (p>0.05). The calcium absorption rates of the middle and high dose groups and the calcium carbonate group were 39.18%, 40.52%, 38.38%, respectively, and the retention rate was 38.80%, 39.27%, 37.58%, respectively, which were significantly higher than the blank control group 36.31, 35.91 (p<0.05), respectively. The high dose group was significantly better than the calcium carbonate group with the same calcium content (p<0.05). In addition, the femoral dry weight and midpoint diameter of the femur were 0.57 (g) and 2.40 (mm), respectively. The proximal, distal and midpoint density of the femur were 0.19, 0.22, 0.17 (g/cm2), respectively. These were higher than that in the blank group (p<0.05) and there was no significant difference from the calcium carbonate group. It indicated that the salmon collagen oligopeptide calcium had a good calcium-absorbing effect and a bone density-enhancing function, which provided a good theoretical foundation for the development and utilization of related new functional foods.
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[基金项目]
国家重点研发计划项目(2016YFD0400604;2017YFE0108800);国家自然科学基金项目(31671963);中山市协同创新中心认定资助项目(2016C1015);宁波市“十三五”海洋经济创新发展示范项目(NBHY-2017-S1)