本实验旨在研究平卧菊三七醇提物对溃疡性结肠炎的改善效果，选用BALB/c雄性小鼠和SD雄性大鼠，通过TNBS-50%乙醇溶液经肠给药，分别建立轻、重度溃疡性结肠炎模型，然后实施平卧菊三七不同剂量及其有效组分单独或组合的给药干预，结合H&E和PAS染色技术，评估结肠损伤的病理变化。结果表明，对比轻度溃疡性结肠炎模型组，低剂量平卧菊三七组糖原含量降低了147.14%，杯状细胞含量升高了15.39%，而高剂量平卧菊三七组糖原含量则降低了178.89%，杯状细胞含量升高了36.77%。对比重度溃疡性结肠炎模型组，平卧菊三七组相比于模型大鼠糖原含量升高了45.00%，杯状细胞含量相比于模型组升高了95.62%。以上结果表明，平卧菊三七对轻、重度溃疡性结肠炎的治疗均有显著效果，可作为治疗溃疡性结肠炎的潜在药物进行深度开发。

The purpose of this experiment was to examine the therapeutic effect of the ethanolic extract from Gynura procumbens against ulcerative colitis. In this experiment, BALB/c male mice and SD male rats were used to establish animal models with mild and severe ulcerative colitis, respectively, through intraintestinal administration of TNBS-50% ethanol solution. Then, different doses of Gynura procumbens extract and its effective components (alone or in combination) were administered separately, and H&E and PAS staining techniques were used to evaluate colonic injury-related pathological changes. The results showed that compared with the mild ulcerative colitis model group, the glycogen content of the low-dose and high-dose Gynura procumbens group decreased by 147.14% and 178.89%, respectively, with the goblet cell content of these two groups increased by 15.39% and 36.77%. Compared with the severe ulcerative colitis model group, the glycogen content of Gynura procumbens group increased by 45.00% with the goblet cells increasing by 95.62%. The Gynura procumbens extract can be used to treat mild and severe ulcerative colitis as a potential drug for further development.

国家自然科学基金资助项目（81673711；81573887）