为了筛选具有降尿酸作用的乳酸菌新菌株，本研究以自主分离鉴定的5株植物乳杆菌（Lactobacillus plantarum）为研究对象，对其体外降解嘌呤核苷能力进行了评价，获得植物乳杆菌UA149是一株降解嘌呤核苷能力较强的菌株，并通过动物体内实验评价了该菌株降尿酸作用和机制。以氧嗪酸钾联合果糖诱导高尿酸血症大鼠模型，植物乳杆菌UA149菌液连续灌胃两周，测定血清中尿酸、黄嘌呤氧化酶和炎症因子水平等相关生化指标，并采用蛋白杂交技术，探讨植物乳杆菌UA149降尿酸的作用机制。结果表明：植物乳杆菌UA149能够显著降低高尿酸血症模型大鼠血尿酸水平（39.71%），降低血清黄嘌呤氧化酶活性（15.36%），降低白三烯（25.81%）、血栓素（38.40%）和炎症因子水平（IL-1 26.89%、IL-8 10.40%、IL-6 17.29%、IL-1β 20.95%和TNF-α 23.09%）。植物乳杆菌UA149可以通过调节AMPK/NF-κB/NLRP3信号通路来减缓高尿酸血症引起的肾脏炎症反应。说明植物乳杆菌UA149可作为益生菌用于高尿酸血症的预防和辅助治疗。

In order to screen Lactobacillus plantarum with assistant uric acid-lowering function, five species of L. plantarum independently isolated and identified by us were evaluated its ability to degrade purine nucleosides in vitro. L. plantarum UA419, a strain with strong degradation ability of purine nucleoside, was further studied. The L. plantarum UA419 was given to the hyperuricemia rats (induced by potassium oxonate combined with fructose) by gavage for continuous 2 weeks. The uric acid, xanthine oxidase and inflammatory factor levels in serum were measured and western bolt was used to explore the mechanism of the strain’s lowering uric acid. The results showed that L. plantarum UA419 could significantly reduce serum indexes, including uric acid (39.71%), xanthine oxidase (15.36%), leukotrienes (25.81%), thromboxane (38.40%) and inflammatory factor levels (IL-1 26.89%, IL-8 10.40%, IL-6 17.29%, IL-1β 20.95% and TNF-α 23.09%). L. plantarum UA419 might be a promising agent for gouty treatment to inhibit renal NF-κB/NLRP3 inflammasome activation via the AMPK/SIRT1 pathway. These results indicated that L. plantarum UA419 plays an assistant uric acid-lowering effect.

吉林省农业科技创新工程重大项目(CXGC2017ZD011)；吉林省农业科学院人才引进启动基金（C7208000426）；国家现代农业（奶牛）产业技术体系建设专项项目（CARS-36）