本研究通过建立高脂血症大鼠模型，利用实验室分离保藏具有较好体外抗氧化能力的植物乳杆菌67，结合“间歇性断食法”，设计了一周Lp 67菌株干预5次的5:2干预组和一周Lp 67菌株隔一天干预一次的隔日干预组，测定干预不同时期血清中GSH-Px、T-SOD、CAT、MDA水平和T-AOC能力，并与其余组进行比较，以探究益生菌干预频次、周期对高脂血症大鼠血清抗氧化能力的作用规律。与模型组相比，干预组均能显著提高血清GSH-Px和T-SOD的水平（p<0.05），显著降低MDA水平（p<0.05），提高T-AOC能力（p>0.05），其中5:2干预组能显著降低CAT水平（p<0.05），隔日干预组能降低CAT水平（p>0.05），综合而言，隔日干预且连续干预8周效果佳，分别提高GSH-Px、T-SOD和CAT水平93.08 nmol/mL、13.05 U/mL和5.97 U/mL，提高T-AOC能力0.50 U/mL，降低MDA水平10.04 nmol/mL。实验结果表明益生菌干预频次、周期对高脂血症大鼠的抗氧化能力有显著性影响。

In this study, a rat model of hyperlipidemia was established, and the Lactobacillus plantarum 67 with high in vitro antioxidant capacity was preserved separately in the laboratory. According to the “intermittent fasting” method, an intervention with the Lp 67 strain being intervened 5 times per week (the 5 : 2 intervention group) or being intervened once per two days (the every other day intervention group) were established. The levels of serum GSH-Px, T-SOD, CAT and MDA and the T-AOC ability of these two groups were measured in different periods of intervention, and compared with those of the other groups, to explore the influence of probiotic intervention frequency and cycle on the serum antioxidant capacity of hyperlipidemic rats. Compared with the model group, the intervention group had significantly increased levels of serum GSH-Px and T-SOD (p<0.05), significantly decreased MDA level (p<0.05), and insignificantly improved T-AOC ability (p>0.05). The CAT level significantly reduced (p<0.05) for the 5:2 intervention group but insignificantly reduced (p>0.05) for the every other day intervention group. In general, the every other day intervention of the Lp 67 strain continuously for 8 weeks was more effective, which increased the GSH-Px level, T-SOD level, CAT level, and T-AOC ability by 93.08 nmol/mL, 13.05 U/mL, 5.97 U/mL, and 0.50 U/mL, respectively, while decreasing the MDA level by 10.04 nmol/mL. The experimental results showed that the frequency and cycle of probiotic intervention had significant effects on the antioxidant capacity of hyperlipidemic rats.

国家自然科学基金项目（31571855）；江苏省高校自然科学研究重大项目（17KJA550004）