“伯谢克辛”甜瓜属于典型的呼吸跃变型果实，在采后3~5 d迅速软化。使用1 μL/L 1-甲基环丙烯（1-methylcyclopropene，1-MCP）和300 mg/kg乙烯利处理“伯谢克辛”甜瓜，研究“伯谢克辛”甜瓜调控软化机理，分析硬度、果胶物质含量、纤维素降解变化规律，纤维素酶（Cx）、β-葡萄糖苷酶（β-Glu）、多聚半乳糖醛酸酶（PG）、果胶甲酯酶（PME）活性变化规律。结果表明：1-MCP处理组延缓了甜瓜硬度的下降，原果胶、纤维素含量显著高于对照及乙烯利处理。原果胶比对照组高22%（p<0.05）、纤维素含量比对照组高26.13%（p<0.05）；1-MCP处理PG、PME、Cx、β-Glu活性显著低于对照及乙烯利处理组。PG、PME、Cx、β-Glu活性均低于对照组，酶活高峰时比对照组低8.92%、25.73%、32.45%、14.82%。乙烯利处理PG、Cx、β-Glu活性均高于对照组，酶活高峰时比对照组高2.84%、21.83%、14.95%。随着贮藏时间的增加，1-MCP处理通过调节“伯谢克辛”甜瓜果胶类物质、纤维素含量及细胞壁降解酶的活性，减缓细胞软化进程，提高“伯谢克辛”甜瓜的贮藏品质。

“Bethekxin” melon is a typical climacteric fruit that softens rapidly 3 to 5 days after harvest. Treatment of “Besekxin” melon with 1 μL/L 1-methylcyclopropene (1-MCP) and 300 mg/kg ethephon to study the softening mechanism of “Bethekxin” melon and analyze the hardness and pectin substance Changes in the content, cellulose degradation, cellulase (Cx), β-glucosidase (β-Glu), polygalacturonase (PG), pectin methylesterase (PME) activity. The results showed that the 1-MCP treatment group delayed the decrease of melon hardness, and the original pectin and cellulose content were significantly higher than that of the control and ethephon treatment. The original pectin was 22% higher than the control group (p<0.05), and the cellulose content was 26.13% higher than the control group (p<0.05). The activity of PG, PME, Cx and β-Glu treated by 1-MCP was significantly lower than that of the control and ethylene. Processing group. The activities of PG, PME, Cx and β-Glu were lower than those of the control group. The peak enzyme activity was 8.92%, 25.73%, 32.45% and 14.82% lower than the control group. The activities of PG, Cx and β-Glu in ethephon treatment were higher than those in the control group, and the peak of enzyme activity was 2.84%, 21.83% and 14.95% higher than that of the control group. With the increase of storage time, 1-MCP treatment slowed the cell softening process by regulating the activity of “Bethekxin” melon pectin, cellulose content and cell wall degrading enzyme, and improved the storage quality of “Bethekxin” melon.

国家自然科学基金项目（3166100317）