探究苦参多糖对人肺癌细胞株增殖、凋亡、侵袭、迁移能力的影响。选取人肺癌A549细胞株，经过培养后分为空白组、药物对照组以及低、中、高浓度组，检测各组细胞增殖、凋亡、侵袭、迁移能力，并对凋亡相关蛋白Bcl-2、PI3K、Akt表达进行测定。研究结果显示，72 h时高浓度组细胞增殖率为18.65%，凋亡率为79.55%，均优于其他各对照组。高浓度组细胞侵袭数、迁移数均低于其他各组（p<0.05）。高浓度组细胞Bcl-2、PI3K、Akt相对表达量分别为0.32、0.40、0.34，均低于其他各组（p<0.05）。在苦参多糖干预下，肺癌细胞增殖率下降，凋亡率上升，侵袭、迁移能力受到明显的抑制，凋亡相关蛋白Bcl-2、PI3K、Akt表达受到明显的调控，且其能力呈现明显的浓度依赖性，说明苦参多糖能够抑制肺癌细胞增殖、侵袭、迁移，促进肺癌细胞凋亡，为肺癌的临床治疗提供一定的理论依据。

To explore the effects of Sophora flavescens polysaccharide on proliferation, apoptosis, invasion and migration of human lung cancer cell lines, human lung cancer A549 cell lines were cultured and divided into blank group, drug control group and low, medium and high concentration groups. Cell proliferation, apoptosis, invasion and migration ability were detected in each group, and the expressions of apoptosis-related proteins Bcl-2, PI3K and Akt were determined. The results showed that the cell proliferation rate and apoptosis rate of the high concentration group, at 72 h, were 18.65% and 79.55% respectively, which were superior to those of the other control groups. The number of cell invasion and migration in high concentration group was lower than that in other groups (p<0.05). The relative expressions of Bcl-2, PI3K and Akt in high concentration group were 0.32, 0.40 and 0.34 respectively, which were lower than those in other groups (p<0.05). Under the intervention of Sophora flavescens polysaccharide, the proliferation rate of lung cancer cells decreased, the apoptosis rate increased, the invasion and migration ability were inhibited obviously, and the expression of apoptosis-related proteins Bcl-2, PI3K and Akt were obviously regulated, and their ability showed obvious concentration dependence. This indicated that Sophora flavescens polysaccharide could inhibit the proliferation, invasion and migration of lung cancer cells, promote apoptosis of lung cancer cells and provide a theoretical basis for clinical treatment of lung cancer.

江苏省自然科学基金资助项目（BK20180273）；江苏医药职业学院科技创新团队资助项目（20188103）；江苏高等学校A类品牌药学专业建设项目（PPZY2015A097）