本实验通过研究丹参酮的浓度和各项指标增减变化，来探讨其对膝骨关节炎的抗炎作用。构建四组佐剂性关节炎大鼠模型，各组给药21 d。通过膝骨关节炎评估标准评及膝骨直径测量确定大鼠膝骨关节炎指数；利用离体血管环灌流与双抗体夹心ABC-酶联免疫吸附法，分别检测各组受乙酰胆碱影响的内皮依赖性舒张功能与血管性血友病因子含量；在丹参酮样本中采集细胞荧光值，通过荧光素酶报告基因活性确定丹参酮抗炎活性。研究结果表明，给药后，丹参酮组膝骨关节炎评估值约为5.26，低于甲氨蝶呤组和枫杨乙醇提物组；注入乙酰胆碱后舒张率约为88.72 η/%，无显著波动；血管性血友病因子含量约为35.22 λB/(U/L)，最接近对比组，9份丹参酮样本中，55%洗脱液的其荧光素酶报告基因活性约为89.26，其抗炎效果最佳，说明丹参酮能够修复内皮细胞，对膝骨关节炎模型大鼠的抗炎作用较好。

In this study, the anti-inflammatory effect of tanshinone on knee osteoarthritis was studied based on the concentration of tanshinone and the changes of various indexes. Four groups of adjuvant arthritis rat models were constructed, each group was given 21 days. Knee osteoarthritis index in rats was determined by knee osteoarthritis evaluation criteria and knee bone diameter measurement. Isolated vascular ring perfusion and double-antibody sandwich abc-enzyme-linked immunosorbent assay were used to determine the content of acetylcholine-influenced endothelium-dependent relaxation function and von willebrand factor in each group. Cell fluorescence values were collected from the samples of tanshinone and the anti-inflammatory activity of tanshinone was determined by the activity of luciferase reporter gene. The results showed that the evaluation value of knee osteoarthritis in tanshinone group was about 5.26, which was lower than that in methotrexate group and fengyang ethanol extract group. After the injection of acetylcholine the relaxation rate was about 88.72 η/%, no significant fluctuations; The vascular haemophiliac factor content was approximately 35.22 λB/U?L-1, the closest to the control group. In the 9 samples of tanshinone, the luciferase reporter activity of 55% eluent was approximately 89.26, with the best anti-inflammatory effect, indicating that tanshinone can repair endothelial cells, and has a good anti-inflammatory effect on knee osteoarthritis model rats.

陕西省教育厅科学研究计划项目（17JK0833）