研究观察玛咖生物碱对小鼠结肠癌CT-26细胞成瘤能力的影响并初步探讨其可能机制。构建BALB/c小鼠CT-26细胞移植瘤动物模型，随机分为玛咖生物碱高剂量组、玛咖生物碱低剂量组及生理盐水模型对照组。连续灌胃用药27 d，绘制小鼠体重增长曲线及移植瘤体积增长曲线。第28 d处死小鼠，计算抑瘤率、相对肿瘤增殖率、脾脏指数；ELISA法检测血清IL-2、IFN-γ水平；HE切片观察移植瘤、脾脏、肝脏及肾脏组织学形态变化。结果表明，与对照组比较，实验组移植瘤重量明显降低（p<0.05），抑瘤率分别为43.37%和45.26%，组织学观察实验组移植瘤坏死面积较对照组明显增大。实验组小鼠脾脏指数明显高于对照组（p<0.05），且实验组脾脏白髓增生较显著。同时，血清IL-2及IFN-γ水平均明显高于对照组（p<0.05）。可见，玛咖生物碱可以通过提高小鼠免疫力抑制CT-26细胞的成瘤。

The effect of maca alkaloids on the tumorigenic ability of mouse colon cancer CT-26 cells was investigated and its possible mechanism was explored. The animal model of colon cancer CT-26 cell transplantation in BALB/c mice was established and randomly divided into high dose maca alkaloid group, low dose maca alkaloid group and saline control group. The drug was continuously administered for 27 days. The weight gain curve of the mice and the volume growth curve of the transplanted tumor were drawn. The mice were sacrificed on the 28th day. The tumor inhibition rate, relative tumor proliferation rate and spleen index were calculated. The levels of serum IL-2 and IFN-γ were detected by ELISA. The histological changes of transplanted tumor, spleen, liver and kidney were observed by HE section. The results showed that, compared with the control group, the weight of the transplanted tumor in the experimental groups was significantly reduced (p<0.05).The tumor inhibition rates of the experimental groups were 43.37% and 45.26%, respectively. Histological observation showed that the necrotic area of transplanted tumor in the experimental group was significantly increased compared with the control group. The spleen index of the experimental group was significantly higher than that of the control group (p<0.05), and the white pulp proliferation of the spleen of the tumor-bearing mice in the experimental group was significantly larger than that in the control group. The serum IL-2 and IFN-γ levels in the experimental group were significantly higher than that in the control group (p<0.05). Results indicated that maca alkaloids could inhibit the tumor formation of mouse colon cancer CT-26 cells by increasing mouse immunity.

省级大学生创新创业训练计划项目（201710632107）