大球盖菇（Stropharia rugoso-annulata）具有很高的营养价值和药用价值，但是目前对于大球盖菇培养基的筛选以及培养条件的优化研究还不够深入。本研究通过测定不同母种培养基配方下大球盖菇的菌丝生长速度、生长势以及菌落生长指数，筛选出大球盖菇最适宜母种培养基；测定不同液体培养基配方下大球盖菇菌丝球的生物量，筛选出最适宜的液体培养基；并在单因素试验的基础上，以大球盖菇菌丝球生物量为响应值，应用Box-Benhnken中心组合法对大球盖菇液体菌种培养条件进行了优化。结果表明：最适宜大球盖菇母种生长的配方为马铃薯200 g、干稻草150 g、蔗糖20 g、玉米粉10 g、蛋白胨3 g、酵母膏2 g、维生素B1 5 mg、琼脂20 g、水1000 mL；最适宜大球盖菇液体种生长的配方为蔗糖20 g、玉米粉30 g、稻草粉15 g、蛋白胨2 g、酵母膏1 g、维生素B1 5 mg、水1000 mL (pH自然)；液体菌种最佳培养条件为转速140 r/min，接种量8%，培养时间12 d，三次平行实验得到的实际的大球盖菇菌丝球生物量平均为0.8108 g/100 mL，与预测值0.8282 g/100 mL相符。

Stropharia rugoso-annulata has high nutritional value and medicinal value, but currently efforts on the screening of its culture medium and optimization of its culture conditions remain insufficient. In this study, the mycelial growth rate, growth potential and colony growth index of Stropharia rugoso-annulata were determined to screen the most suitable mother seed medium. The mycelial biomass was determined under different liquid medium formulas to screen the most suitable liquid medium for Stropharia rugoso-annulata. Based on the single-factor tests, the mycelial biomass of Stropharia rugoso-annulata was used as the response value, and the Box-Benhnken and central-composite method was applied to the optimization of liquid culture conditions for Stropharia rugoso-annulata. The results showed that the most suitable formula of the mother seed medium for the growth of Stropharia rugoso-annulata was: potato 200 g, dried rice straw 150 g, sucrose 20 g, corn flour 10 g, peptone 3 g, yeast extract 2 g, vitamin B1 5 mg, and water 1000 mL; The most suitable formula of the liquid medium for the growth of Stropharia rugoso-annulata was: sucrose 20 g, corn flour 30 g, straw powder 15 g, peptone 2 g, yeast extract 1 g, vitamin B1 5 mg, and water 1000 mL (natural pH); The optimal culture conditions for the liquid strain were: rotation speed 140 r/min, inoculum quantity 8%, incubation time 12 d. The mean of the actual mycelial biomass values obtained from three parallel experiments was 0.8108 g/100 mL, which was in agreement with the predicted value (0.8282 g/100 mL).

中央高校基本科研业务费专项资金项目（2572017CF01）