本研究为明确混合多糖配伍使用抑制皮肤细胞衰老的作用是否优于单一多糖，通过利用不同浓度的H2O2诱导人皮肤成纤维细胞（HSF）、人角质形成细胞（HaCaT）出现损伤老化，在此基础上，采用MTT法和生化试剂盒检测，分别检测细胞的存活率及超氧化物歧化酶（SOD）、丙二醛（MDA）、羟脯氨酸（HYP）的含量。本实验所建立的HaCaT及HSF细胞过氧化模型的存活率分别约为72.00%和65.00%，研究发现四种单一多糖组对两种细胞老化模型的存活率与模型组相比差异没有统计学意义（p>0.05）；其中SOD、MDA、HYP的含量与模型组相比，除个别指标外，差异没有统计学意义（p>0.05）。在HaCaT细胞老化模型中，混合多糖组的存活率在74.93%~80.43%之间，SOD含量在6.99 U/mg~7.56 U/mg之间，MDA含量在7.44 μmol/g~8.41 μmol/g之间，HYP含量表达在2.30 μmol/g~2.43 μmol/g之间；在HSF细胞老化模型中，混合多糖组的存活率在76.23%~78.14%之间，SOD含量在6.30 U/mg~8.90 U/mg之间，MDA含量在7.03 μmol/g~8.93 μmol/g之间，HYP含量在2.19 μmol/g~2.53 μmol/g之间；以上指标与模型组相比差异均有统计学意义（p<0.01或p<0.05）。因此，混合多糖组的整体改善效果更为显著，它能够提高衰老表皮细胞生存率，提高模型细胞SOD、HYP含量，使MDA含量降低。研究提示我们在对抗H2O2诱导的HSF、HaCaT细胞氧化衰老实验中，在相同干预浓度下，不同多糖配伍后的混合多糖比组分单一多糖单独使用效果显著。

The purpose of this study was to determine whether the effect of mixed polysaccharides in inhibiting skin cell senescence is superior to that of single polysaccharides by using different concentrations of H2O2 to induce damage to human skin fibroblasts (HSF) and human keratinocytes (HaCaT), on this basis, MTT assay and biochemical kit were used to detect cell viability and superoxide dismutase (SOD), malondialdehyde (MDA) and hydroxyproline (HYP). The survival rates of HaCaT and HSF cell peroxidation models established in this experiment were about 72.00% and 65.00%, respectively. The study found that the survival rate of the two single aging models was not statistically different from that of the model group (p>0.05). The contents of SOD, MDA and HYP were not significantly different from those of the model group except for individual indicators (p>0.05). In the HaCaT cell aging model, the survival rate of the mixed polysaccharide group was between 74.93% and 80.43%, the content of SOD was between 6.99 U/mg and 7.56 U/mg, the content of MDA was between 7.44 μmol/g and 8.41μmol/g, and the content of HYP was between 2.30 μmol/g and 2.43 μmol/g. In the HSF cell aging model, the survival rate of the mixed polysaccharide group was between 76.23% and 78.14%, the content of SOD was between 6.30 U/mg and 8.90 U/mg, the content of MDA was between 7.03 μmol/g and 8.93 μmol/g, and the content of HYP was between 2.19 μmol/g and 2.53 μmol/g. The above indicators were statistically significant compared with the model group (p<0.01 or p<0.05).Therefore, the overall improvement effect of the mixed polysaccharide group was more significant, which can improve the survival rate of senescent epidermal cells, increase the SOD, HYP content and reduce the MDA content of model cells. The study suggested that in the anti-H2O2-induced HSF and HaCaT cell oxidative senescence experiments, the mixed polysaccharides of different polysaccharides were significantly more effective than the single polysaccharides alone, at the same intervention concentration.

国家中医药管理局第四批全国中医优秀人才研修项目（国中医药人教发[2017]24号）；广东省中药新药研发重点实验室（2017B030314096）；广东省省级科技计划科技基础条件建设领域项目（2014A030304059）；广东省省级科技计划科技应用型科技研发专项资金项目（2015B020234008）