本研究从8份养殖水体样品中富集、分离、纯化、鉴定能脱除亚硝态氮、硝态氮和氨氮的菌株资源，获得8株亚硝态氮脱除菌、8株硝态氮脱除菌和11株氨氮脱除菌，分布在Pseudomonas、Paracoccus、Zobellella、Achromobacter、Bosea、Paenarthrobacter、Corynebacterium、Bacillus、Delftia和Gordonia等属。对这27株菌进行脱氮特性分析，筛得2株异养硝化-好氧反硝化菌，分别鉴定为Pseudomonas stutzeri j-1和Zobellella denitrificans2G-5。这2株菌脱氮性能优越，在3种氮素培养基中培养18 h后，对亚硝态氮、硝态氮和氨氮的脱除率在99.53%~99.89%之间。将之用于未灭菌的养殖水脱氮，菌株j-1表现出一定的定殖和增殖能力，发挥出良好的脱氮效果；但菌株2G-5增殖能力较弱，脱氮效果不明显。本研究发掘了一批脱氮菌株资源，获得了2株具有重要应用潜力的异养硝化-好氧反硝化菌，为细菌氮循环代谢机制研究和脱氮技术研发积累了物质基础。

The present study collected 8 samples from aquaculture ponds for performing enrichment, isolation, purification and identification of bacteria strain scapable of removing NO2--N, NO3--N and NH4+-N. We obtained 8, 8 and 11 bacteria strains which could remove NO2--N, NO3--N and NH4+-N, respectively. These bacteria were classified into the genera Pseudomonas, Paracoccus, Zobellella, Achromobacter, Bosea, Paenarthrobacter, Corynebacterium, Bacillus, Delftia and Gordonia. After analysis of denitrification characteristics, two heterotrophic nitrification and aerobic denitrification bacteria strains with excellent denitrification effects were obtained and identified as Pseudomonas stutzeri j-1 and Zobellella denitrificans 2G-5, respectively. After incubated in 3 nitrogen media for 18 hours, the removal rates of NO2--N, NO3--N and NH4+-N by strain j-1 and 2G-5 were ranged from 99.53% to 99.89%. When applied in non-sterile aquaculture water, strain j-1 was successfully colonized and showed efficient denitrification. Whereas, strain 2G-5 didn’t proliferate well and its denitrification effect was unremarkable. This study exploited a collection of denitrification bacteria, obtained two heterotrophic nitrification and aerobic denitrification strains with important application potential, and provided biological materials for further study on bacterial mechanisms of nitrogen circulation and metabolism, and on research and development of denitrification techniques.

广东省科技计划项目（2016A020210057；2018A030313476）；广州市珠江科技新星专项（201806010065）；广东省科学院项目（2019GDASYL-0401002）；广东省科技创新领军人才项目（2015TX01N036）；广东省农业厅项目（2016LM3158）；广州市科技计划项目（201704020088）；肇庆市科技计划项目（2018N003）