[关键词]
[摘要]
采用电子鼻(E-Nose)结合顶空固相微萃取-气质联用(HS-SPME-GC-MS)技术对四种不同生境模式(池塘、稻田、湖泊、长江)的雄性中华绒螯蟹性腺挥发性成分进行鉴定。电子鼻结果显示,湖泊和稻田雄蟹性腺风味轮廓相似,而池塘和长江雄蟹性腺的风味轮廓较为独特,可明显区分。运用GC-MS对四种雄蟹性腺中挥发性成分的种类和含量进行分析,并通过各挥发性成分的阈值计算其气味活度,筛选出主体呈香化合物(OAV≥1)。结果表明,湖泊和稻田雄蟹性腺中的挥发性成分在组成和含量上相近,而池塘和长江雄蟹性腺中的挥发性成分则比较复杂,且长江雄蟹性腺中己醛(634.36 ng/g)、苯甲醛(1361.98 ng/g)和2-戊基呋喃(231.11 ng/g)等主体呈香化合物的含量明显高于其他三种雄蟹性腺。综上结果表明,湖泊和稻田雄蟹性腺的风味相似,而池塘和长江雄蟹性腺的风味比较独特,且在四种模式中,长江雄蟹性腺风味相对较优。
[Key word]
[Abstract]
Both E-Nose and HS-SPME-GC-MS techniques were applied to identify volatile components from gonad of male Eriocheir sinensis cultured with four different eco-environment modes (pond, rice-field, lake, and Yangtze River). The results of E-Nose showed that the flavor profiles of gonad in male Eriocheir sinensis cultured in lake and rice-field were similar, while the flavor profiles of gonad from pond and Yangtze River were unique and could be distinguished well. GC-MS was used to analyze the type and content of volatile components in male gonad, and the main aroma compounds were selected according to their thresholds (OAV≥1). The results showed that the volatile components in the gonad of lake and rice-field male crab were similar in composition and content, but in the gonad of pond and Yangtze male crab, the composition of volatile components was more complicated, and the contents of hexanal (634.36 ng/g), benzaldehyde (1361.98 ng/g), and 2-pentylfuran (231.11 ng/g) in the gonad of male crab from the Yangtze River were significantly higher than those in the other three male gonad. In conclusion, this study showed that the flavors of gonad in male Eriocheir sinensis cultured in lake and rice-field were similar, while the flavors of gonad from pond and Yangtze River were unique, and among the four different modes, the gonad of Yangtze River has better flavor than others.
[中图分类号]
[基金项目]
国家虾蟹产业技术体系(CARS-48);安徽水产产业技术体系(AHCYJSTX-08)