本文旨在研究桑叶生物碱对高脂饮食小鼠抗氧化能力的影响。以含桑叶生物碱剂量分别为20、40和80 mg/kg灌胃喂饲高脂饮食小鼠4、8、12、16周，用试剂盒测定各组小鼠肝脏和血清中丙二醛(malondlaldehyde，MDA)等指标。结果表明，与高脂对照组小鼠相比，以桑叶生物碱剂量为80 mg/kg灌胃小鼠16周，小鼠肝脏中MDA含量减少43.63%，血清中的减少50.91%；肝脏中总抗氧化能力(total antioxidant capability，T-AOC)、还原型谷胱甘肽(glutathione，GSH)含量分别增加2.36倍、2.69倍，血清中的分别增加2.25倍、1.59倍；肝脏中过氧化氢酶(catalase，CAT)、过氧化物酶（peroxidase，POD）、谷胱甘肽过氧化物酶(glutathione peroxidase，GSH-Px)、超氧化物歧化酶(superoxide dismutase，SOD)活性分别增加1.82倍、2.22倍、1.90倍、1.22倍，血清中的分别增加1.48倍、2.19倍、1.59倍、1.44倍。结论，桑叶生物碱对高脂饮食小鼠具有较强的抗氧化作用。

To study the antioxidant capacity of mulberry alkaloid in mice on high fat-diet, the mice on high fat-diet were fed with alkaloid at different concentrations (20, 40, 80 mg/kg) by gastric gavage for 4, 8, 12, 16 weeks respectively, the content of malondialdehyde (MDA) and other indicators in liver and serum of the mice on high fat-diet were assessed by kit. The results showed that in the mice fed with a dose of 80 mg/kg by gastric gavage for 16 weeks, the content of MDA in liver decreased by 43.63% and in serum decreased by 50.91%, compared with the hyperlipidemic control group. The contents of T-AOC and GSH in liver were increased by 2.36 and 2.69 times respectively, and the contents in serum increased by 2.25 and 1.59 times respectively. The activity of glutathione (GSH), catalase (CAT), peroxidase (POD), glutathione peroxidase (GSH-Px) and superoxide dismutase(SOD) in liver increased by 1.82, 2.22, 1.90 and 1.22 times, and the activity in serum increased by 1.48, 2.19, 1.59 and 1.44 times, respectively. In conclusion, the alkaloids of mulberry leaves have strong antioxidant effects on high-fat diet mice.

重庆市科委社会民生重点项目（CSTS2017shms-zdyfx0054）；公益性行业（农业）科研专项（201403049）；国家现代农业（桑蚕）产业技术体系建设专项（CARS-18）