研究了咖啡酸苯乙酯(caffeic acid phenethylester，CAPE)对2型糖尿病大鼠胰脏的保护作用及其作用机制。将30只雄性SD大鼠随机分为3组，即空白组，损伤组，保护组。以高脂高糖饲料喂养联用链脲佐菌素(Streptozocin，STZ)诱导建立2型糖尿病大鼠模型，保护组给予CAPE保护，建模成功后，测定其胰脏中丙二醛(Malondialdehyde，MDA)、蛋白羰基化(Protein carbonylation，PCO)、一氧化氮(Nitric oxide，NO)、谷胱甘肽(Glutathione，GSH)的含量及超氧化物歧化酶(superoxide dismutase，SOD)、过氧化氢酶(Catalase，CAT)的活性。实验结果显示，损伤组中MDA、PCO、NO含量明显升高，分别为空白组的2.52倍、1.64倍以及2.78倍，而保护组MDA、PCO、NO的含量较损伤组减少，仅为损伤组的47%、69%以及47%。说明糖尿病能够导致胰脏的氧化应激，破坏其结构与功能，给予CAPE保护可以使MDA、PCO、NO的含量降低，减少对胰脏的破坏；机体中SOD、CAT、GSH等抗氧化物质活性因机体应对氧化应激产生的过量自由基而降低，CAPE可增强SOD、CAT、GSH的活性，加快清除体内自由基的速度，对糖尿病胰脏有一定的保护作用。

The protective effect of caffeic acid phenethylester (CAPE) on pancreas of type 2 diabetic rats and their mechanism were investigated. Thirty male SD rats were randomly divided into three groups: blank group, injury group and protection group. The rat model of type 2 diabetes mellitus was induced by high-fat and high-sugar diet combined with streptozocin (STZ). The protective group was protected by CAPE. The contents of malondialdehyde (MDA), protein carbonylation (PCO), nitric oxide (NO), glutathione (GSH) and activities of super oxide dismutase (super oxide dismutase), catalase (CAT) in pancreas were determined. The results showed that the contents of MDA, PCO and NO in the injured group were significantly increased, which were 2.52, 1.64 and 2.78 times higher than those in the blank group, respectively, while the contents of MDA, PCO and NO in the protective group were lower than those in the injured group, only 47%, 69% and 47% of the injured group, respectively. It indicated that diabetes can lead to oxidative stress of pancreas and destroy its structure and function. Protecting with CAPE could decrease the contents of MDA, PCO and NO and reduce the damage to pancreas. The activities of SOD, CAT, GSH and other antioxidants in the body were reduced by excessive free radicals produced by the body in response to oxidative stress. CAPE could enhance the activities of SOD, CAT and GSH and accelerate the speed of scavenging free radicals in the body. It had certain protective effect on diabetic pancreas.

陕西省科技厅一般项目-农业领域（2017NY-103）；农业部农产品加工重点实验室开放基金（2017KF-07）