为研究广东省水生动物中的维氏气单胞菌（Aeromonas veronii）和无乳链球菌（Streptococcus agalactiae）对不同种类抗生素的药物敏感性，从广东省9个地区人工养殖的水生动物体内分离维氏气单胞菌及无乳链球菌。其中分离出32株维氏气单胞菌和16株无乳链球菌。利用药敏纸片法检测分离菌株对14种抗生素的药物感受性，发现两种病菌对其中的7种抗菌药物有明显的敏感性。利用最小抑菌浓度法检测分离菌株对7种敏感抗生素的耐药性，发现32株维氏气单胞菌对甲砜霉素（29%）部分耐药，盐酸土霉素（41%）和交沙霉素（38%）部分敏感，对盐酸环丙沙星（82%）、强力霉素（82%）、氟苯尼考（75%）和磺胺二甲嘧啶（100%）都敏感。16株链球菌对甲砜霉素（100%）全部耐药，对交沙霉素（25%）部分敏感，对盐酸环丙沙星（100%）、强力霉素（100%）、盐酸土霉素（100%）、氟苯尼考（100%）和磺胺二甲嘧啶（88%）都敏感。本论文的研究可以对今后抗生素在维氏气单胞菌与无乳链球菌的使用中提供一定的参考，促进抗生素的合理使用。

To evaluate the antibiotic sensitivity of Aeromonas veronii and Streptococcus agalactiae from aquatic animals in Guangdong Province, Aeromonas veronii and Streptococcus agalactiae were isolated from aquatic animals cultured in 9 areas of Guangdong Province. Among them, 32 strains of Aeromonas veronii and 16 strains of Streptococcus agalactiae were isolated. The sensitivity of these strains to 14 different antibiotics was determined using drug-sensitive disk method. It was found that the two strains of bacteria were sensitive to 7 kinds of antibiotics. Based on the detection of drug resistance of isolated strains to 7 sensitive antibiotics by minimum inhibitory concentration(MIC) method. 32 strains of Aeromonas veronii were found to be resistant to thiamphenicol (29%), sensitive to oxytetracycline hydrochloride (41%) and josamycin (38%), ciprofloxacin hydrochloride (82%), doxycycline hydrochloride (82%), florfenicol (75%) and sulfamethazine (100%). All of 16 Streptococcus agalactiae strains were found to be resistant to thiamphenicol (100%), but sensitive to josamycin (25%), and ciprofloxacin hydrochloride (100%), doxycycline hydrochloride (100%), oxytetracycline hydrochloride (100%), florfenicol (100%) and sulfamethazine (100%). This study provides some reference for the future use of antibiotics in Aeromonas veronii and Streptococcus agalactiae, and promotes the rational use of antibiotics.

国家重点研发计划项目（2017YFF0104904）