本文采用动物和细胞实验开展脂清爽保健茶（ZQS）降血脂作用研究，为降血脂保健食品的开发提供科学依据。参照《辅助降血脂功能评价方法》开展动物实验。实验期间，空白组给予基础饲料，其余大鼠根据TC水平随机分为模型组、阳性对照组、ZQS高、中、低剂量组，给予高脂饲料。各组大鼠正常饲养，ZQS高、中、低剂量及阳性药灌胃给药4周后，测定各组大鼠血清中TC、TG、LDL-C、HDL-C含量；细胞实验中，研究ZQS对油酸钠诱导的hepG2细胞高脂模型中TC、TG含量的影响，进行统计分析，评价ZQS的降血脂作用。结果表明，与模型组相比，ZQS高、中、低剂量组大鼠血清中TC、TG、LDL-C含量显著降低，各指标下降最大幅度为34.52%，43.22%，44.89%。ZQS高剂量组血清中HDL-C升高50.62%；ZQS高、中、低剂量组中细胞内TC、TG含量显著下降，其下降最大幅度为27.54%，27.98%。上述研究结果明确了ZQS的辅助降血脂作用，为ZQS保健食品的开发应用奠定了基础。

This paper was to study the hypolipidemic effect of Zhi Qing Shuang (ZQS) in rats and cells, which provided a reference for the development of health food with hypolipidemic effect. The animal experiment was carried out according to evaluation method of auxiliary hypolipidemic function. During the experiment, 60 rats were used in this study, of which 10 rats as the normal group were given normal feed. The remaining rats were given high-fat feed, according to the TC level, which were randomly divided into model group, positive control group and ZQS (high, middle and low dose) group. Each group was normally fed for 4 weeks, the groups were given ZQS (high, middle and low dose) and positive drug, the levels of total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) were determined after the last gavage administration by collecting the blood of orbit. For in vitro cell experiment, the hyperlipidemia model was established in hepG2 cells which was induced by sodium oleate. The hypolipidemic effect was evaluated by comparing the content of TC, TG between model group and drug group. Compared with the model group, the results showed that ZQS induced a significant reduction in TC, TG and LDL-C levels, with the largest reduce rates of 34.52%, 43.22% and 44.89%, respectively. The content of HDL-C was significant increased up to 50.62% in high dose of ZQS group. The in vitro cell experiment results demonstrated that ZQS induced a significant reduction in TC, TG, with the largest reduce rates of 27.54%, 27.98%. In conclusion, ZQS has the function of reducing blood lipid. The study lays a foundation for the development and application of ZQS health food.

北京中医药大学新奥奖励基金（1000062720062/008/006）