本研究探讨了皱纹盘鲍（Haliotis discus hannai）内脏脂质（Haliotis discus hannai visceral lipid，HDHL）对人肝癌细胞HepG2的脂质代谢的影响，对HDHL脂肪酸成分、HepG2细胞活性、细胞内胆固醇和甘油三酯含量以及脂肪酸代谢相关mRNA基因的表达情况进行了研究。研究发现HDHL中不饱和脂肪酸占总脂肪酸含量59.50%，其中多不饱和脂肪酸占29.71%。HDHL与HepG2细胞共孵培养24 h及48 h后，浓度为0~240 μg/mL的HDHL对HepG2细胞无毒性影响。30~240 μg/mL HDHL可显著降低HepG2总胆固醇含量，30~120 μg/mL HDHL共孵HepG2细胞后，细胞内甘油三酯含量显著降低。qPCR结果显示HDHL可显著降低HepG2细胞脂肪酸合成相关基因SREBP1c、ACC1、FAS和脂肪酸转运吸收相关基因CD36 mRNA水平的表达，提高线粒体脂肪酸氧化基因CPT1的表达。因此，HDHL可能通过抑制脂肪酸合成和脂肪酸转运，增强线粒体中脂肪酸氧化等途径有效调节细胞脂质代谢，该研究为脂质调节功能食品的开发提供理论依据。

In this study, the effects of the visceral lipids from Haliotis discus hannai (HDHL) on lipid metabolism of human hepatoma cell line, HepG2 cells, were investigated. The HDHL fatty acid composition, HepG2 cell activity, and the content of intracellular cholesterol and triglycerides as well as the mRNA expression of fatty acids metabolism-related genes were examined. It was found that the unsaturated fatty acids in HDHL accounted for 59.50% of the total fatty acids, of which polyunsaturated fatty acids accounted for 29.71%. After an incubation of HDHL and HepG2 cells for 24 h and 48 h, respectively, HDHL at 0~240 μg/mL had no toxic effect on HepG2 cells, whereas, HDHL at 30~240 μg/mL could significantly reduce the total cholesterol content of HepG2, with HDHL at 30~120 μg/mL causing a significant decrease in the content of intracellular triglycerides. The qPCR results revealed that in HepG2 cells, HDHL could significantly reduce the mRNA expression of fatty acid synthesis-related genes SREBP1c, ACC1 and FAS as well as fatty acid transport and absorption-related gene CD36, while increasing the expression of mitochondrial fatty acid oxidation gene CPT1. Therefore, HDHL might regulate cell lipid metabolism through inhibiting the synthesis and transportation of fatty acids while enhancing the oxidation of fatty acids in mitochondria. This study provides a theoretical basis for the development of lipid-regulating functional foods.

海洋公益性行业科研专项（201405016）