建立一种同时测定不同食品基质中柠檬黄、日落黄、新红、苋菜红、靛蓝、胭脂红、诱惑红、亮蓝、赤藓红、酸性红十种人工合成着色剂的分离分析方法。对比Eclipse XDB C18、Venusil XBP C18及Symmetry C18三种不同色谱柱对十种着色剂的分离情况，研究Sep-Pak Plus QMA、ProElut PWA-2及Cleanert PWAX-SPE三种固相萃取小柱的回收率及不同食品基质的前处理提取方法。本方法在27 min内可以实现十种人工合成着色剂的分离分析，在0.20~50.00 μg/mL的范围内有良好的线性关系(R2>0.999)，标准混合物在样品中的回收率范围为69.71%~103.80% (n=6)，相对标准偏差范围为0.72%~9.41% (n=6)。本方法前处理简便高效，准确度高，重现性好，回收率、检出限及精密度均能达到国家标准要求，假阴性率底，适用于基层实验室大批量多种基质食品中人工合成着色剂的筛查检测。

To establish a separation and analysis method for the simultaneous determination of 10 kinds of synthetic colorants, such as lemon yellow, sunset yellow, new red, amaranth, indigo, carmine, tempting red, bright blue, red peony and acid red. Comparing the separation of 10 colorants with Eclipse XDB-C18, Venusil XBP C18 and Symmetry C18 columns, the recovery rates of three solid phase extraction cartridges on Sep-Pak Plus QMA, ProElut PWA-2 and Cleanert PWAX-SPE for ten kinds of Synthetic colorants were characterized for qualitative and quantitative purposes. This method could realize the separation and analysis of ten synthetic colorants in 27 min. It has a good linear relationship in the range of 0.20~50.00 μg/mL (R2>0.999). The recovery ratio of the standard mixture was 69.71%~103.80% (n=6) in the samples, relative standard deviation range was 0.72%~9.41% (n=6). The pretreatment of this method was simple and efficient, with high accuracy and good reproducibility. The recovery rate, detection limit and precision could meet the requirements of national standards. The false negative rate was suitable for large-scale matrix foods in grassroots laboratories.

国家自然科学基金资助项目（61704157）