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[摘要]
为研究红树莓对人肝细胞脂肪积累的影响,本文以红树莓果和籽的提取物为原料,测定了提取物中的活性成分,并通过体外油酸诱导建立HepG2细胞脂肪积累模型,以噻唑兰染色吸光度(OD值)、甘油三酯(TG)含量、细胞内脂质含量三个指标,探索红树莓果和籽提取物对肝细胞脂肪积累的影响。试验结果表明:红树莓果提取物中总酚15.18±0.94 mg GAE/g、黄酮1.25±0.05 mg RE/g、原花青素20.35±1.06 mg CE/g、花色苷1.15±0.70 mg CGE/g;红树莓籽提取物中总酚15.35±1.55 mg GAE/g、黄酮11.22±0.66 mg RE/g、原花青素27.08±0.99 mg CE/g、花色苷未检出;以牛血清白蛋白(BSA)作为溶剂配制的1000 μmol/L油酸造模剂孵育HepG2细胞24 h可成功诱导肝细胞脂肪积累;红树莓提取物能显著降低1000 μmol/L油酸诱导的肝细胞脂肪积累,其中200 μg/mL红树莓果提取物处理细胞时,与模型组相比,胞内脂质含量降低了25.93%,TG含量降低了37.23%;4 mg/mL红树莓籽提取物处理时,胞内脂质含量降低了47.12%,TG含量降低了46.23%。红树莓提取物中富含活性成分,能够降低高脂细胞模型内脂肪的积累,对脂肪肝等疾病具有一定的预防作用。
[Key word]
[Abstract]
In order to study the effect of red raspberry on human liver cell fat accumulation, the active components in the extracts of raspberry and seed were determined, and the fat accumulation model of HepG2 cells was established by oleic acid induction in vitro. The results showed that the contents of total polyphenols, flavonoid, proanthocyanidins and anthocyanins in red raspberry extract were 15.18±0.94 mg GAE/g, 1.25±0.05 mg RE/g, 20.35±1.06 mg CE/g, and 1.15±0.70 mg CGE/g, respectively. In raspberry seed extract, the contents of total polyphenols, flavonoid, and proanthocyanidins were 15.35±1.55 mg GAE/g, 11.22±0.66 mg RE/g, and 27.08±0.99 mg CE/g, respectively, and no anthocyanins was detected. Liver cell fat accumulation could be successfully induced by incubating HepG2 cells for 24 h with 1000 μmol/L oleic acid moulding agent prepared with bovine serum albumin (BSA) as solvent. Red raspberry extract could significantly reduce hepatocyte fat accumulation induced by 1000 μmol/L oleic acid. When 200 μg/mL red raspberry extract induced cells, the intracellular lipid content decreased by 25.93% and TG content decreased by 37.23% in comparison with the model group. When treated by 4 mg/mL raspberry seed extract, the intracellular lipid content was decreased by 47.12%, and TG content was decreased by 46.23% in comparison with the model group. Red raspberry extract is rich in active ingredients, which can reduce the accumulation of fat in the high-fat cell model, and has a certain preventive effect on diseases such as fatty liver.
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[基金项目]
中央高校基本科研业务费专项基金(2572017CA05);哈尔滨市应用技术研究与开发项目(2017RAYXJ012)