通过合成2种高核钼簇(NH4)12[Mo36O108(NO)4(H2O)16].33H2O (Mo36)和[Mo36O112(OH2)16(H2bipy)4].28H2O (Mo36-bipy)，其对酪氨酸二酚酶活性的抑制作用机理以及对凡纳滨对虾的防黑变保鲜效果进行研究。结果表明Mo36和Mo36-bipy对酪氨酸二酚酶IC50值分别为0.0358 mmol/L和0.0601 mmol/L，Mo36对酪氨酸酶的抑制作用为可逆竞争性抑制，Mo36-bipy对酪氨酸酶的抑制作用为可逆混合性抑制，且Mo36对酪氨酸酶活性的抑制作用效果优于Mo36-bipy。将凡纳滨对虾在4 ℃环境下贮藏10 d，通过对感官评分、颜色、pH值、TVB-N和菌落总数等指标检测，发现高核钼簇对样品具有一定保鲜效果。其均能适当提升感官分值、L*值，降低pH值与菌落总数。本研究结果为开发一种新型的抗虾体黑变的保鲜剂提供实验基础，并对多金属氧酸盐的应用研究拓展新的研究领域。

Two kinds of high-nuclear molybdenum clusters (NH4)12[Mo36O108(NO)4(H2O)16]?33H2O and [Mo36O112(OH2)16 (H2bipy)4]?28H2O were synthesized. Inhibition mechanisms on diphenolase activity of tyrosianase, anti-melanosis effects of Litopenaeus vannamei and preservation of Mo36 and Mo36-bipy were investigated. The result showed that the IC50 values of tyrosine diphenolase respectively were 0.0358 mmol/L and 0.0601 mmol/L. The inhibitory effect of Mo36 on tyrosinase was reversible competitive inhibition, the inhibition of tyrosinase by Mo36-bipy was reversible mixed inhibition, and the inhibitory effect of Mo36 on tyrosinase activity was better than that of Mo36-bipy. Litopenaeus vannamei was stored under 4 ℃ environment for 10 days, and the sensory score, color, pH value, TVB-N and total number of colonies was detected. It was found that the high-nuclear molybdenum clusters had a certain preservation effect on Litopenaeus vannamei (p<0.05). Mo36 and Mo36-bipy can significantly improve the value of sensory score and L*, and reduce the pH value and the total number of colonies. This paper can provide experimental basis for the preservation of the Litopenaeus vannamei body and the application of polyoxometalates to new areas.

国家自然科学基金项目（21871110）