为了判定木棉花对人体长期服用的安全性，并为以后的食用安全提供毒理学安全性的依据。采用食品安全国家标准中的急性毒性试验、Ames试验、小鼠骨髓细胞微核试验、小鼠精子畸形试验和经口给药方式进行90 d喂养试验。木棉花雌雄性小鼠经口最大耐受剂量（MTD）＞80.00 g/kg·BW；Ames试验、小鼠骨髓细胞微核试验和小鼠精子畸形试验三项遗传毒性试验结果均为阴性。大鼠喂养90 d后，受试样品高剂量组进食量和食物利用率和正常对照组比较有差异，其它各项指标无显著性差异；低、中剂量组SD大鼠体重、进食量、食物利用率、血液学、血液生化、脏器重量以及脏体比等指标与正常对照组比较，均无显著性差异。各主要脏器系数及病理组织学检查比较，均未发现有生物学意义的改变。现有试验结果证明木棉花在低于40.0 g/kg·BW内对人体长期使用是安全可靠的。

To assess the safety of Morinda citrifolia for long-term oral administration and provide the basis of the toxicological safety for future intake. A 90-day feeding trial was conducted using the acute toxicity test，the Ames test, the micronucleus test of bone marrow cells in mice and the sperm shape abnornality test of the national food safety standard. The maximal tolerated dose (MTD) for oral administration of powdered Bombax flower by the male and female mice were＞80.00 g/kg body weight (BW). The results of the three genetic tests, the Ames test,the micronecleus test of bone marrow cells in mice and the sperm shape abnormality test in mice, were all negative. After 90 days of feeding, the food intake and food utilization rate of the high-dose group different from the normal control group, but no significant difference was detected in other indices; the low and medium dose group of SD rats caused insignificant differences in the body weight, food intake, food utilization rate,hematology, blood biochemistry, organ weight and relative ratio of organ to body from the normal control group. No significant changes in biological meaningfulness were found in the major organ coefficients and histopathological examinations. The results obtained in the current research showed that long-term intake of kapok is safe for humans in a dose of less than 40.0 g/kg BW.

海南省自然科学基金项目（20158365）