红松种籽衣中含有多酚、黄酮等活性成分，松籽加工中烘焙工艺会影响籽衣中活性成分含量及抗氧化活性。本研究分别对带壳松籽和不带壳松籽进行烘焙，在130 ℃条件下烘焙10、20、30、40 min，分析其种籽衣中总多酚、黄酮含量和颜色变化，并对其抗氧化能力进行评价。结果表明：烘焙后总多酚、总黄酮含量均低于未焙烤组。不带壳烘焙种籽衣的抗氧化能力强于带壳样品。种籽衣的DPPH·清除能力在不带壳烘焙20 min时最强，IC50为0.16 mg/mL，低于未烘焙组0.17 mg/mL；种籽衣的羟基自由基清除能力在不带壳烘焙20 min时最强，IC50为0.10 mg/mL，低于未烘焙组0.14 mg/mL。L*值、a值、b值随着烘焙时间均呈降低趋势。总多酚和总黄酮含量与 DPPH·清除能力和羟基自由基清除能力正相关，相关系数分别为 0.799和 0.648。种籽衣提取物最大吸收波长在274～279 nm之间。烘焙加工影响红松松籽衣中活性成分含量、抗氧化活性和颜色。

The red pine seed coat contains bioactive components such as polyphenols and flavonoids, and the roasting procedure for processing the pine seed will affect the bioactive contents and antioxidant activity in pine nut seed coat. In this study, both shelled and in-shell pine seed were roasted at 130 ℃ for 10, 20, 30, and 40 min, before the total phenolic content, total flavonoid content, colour change, and antioxidant activity of the seed coat were determined The results showed that the contents of total polyphenols and total flavonoids after roasting were both lowered. The antioxidant ability of the coat of the shelled seeds was higher than that for the in-shell ones. The DPPH scavenging capacity of the shelled seed coat reached the highest after 20-min roasting, with an IC50 of 0.16 mg/mL (which was lower than that for the unroasted ones, 0.17 mg/mL). The hydroxyl free radical scavenging capacity of the shelled roasted seed coat reached the highest after 20 min, with an IC50 of 0.10 mg/mL (which was lower than that for the non-roasted ones, 0.14 mg/mL). The L* value, a value and b value all decreased with the roasting time. The total polyphenol content and total flavonoid content were positively correlated with the DPPH scavenging capacity and hydroxyl radical scavenging capacity, with the correlation coefficients as 0.799 and 0.648, respectively. The peak absorption for the seed coat extract occurred between 274 and 279 nm. Thus, roasting influences the bioactive contents, antioxidant activity and color of the pine seed coat.

教育部东北林业大学大学生创新训练计划项目（201710225283）；哈尔滨市应用技术研究与开发项目科技创新人才专项（2017RALXJ001）