[关键词]
[摘要]
本研究旨在探究不同生长温度(12 ℃、25 ℃、37 ℃)、培养基质(胰蛋白胨大豆肉汤、婴幼儿乳粉肉汤)和接触材料(不锈钢、玻璃、硅胶)对阪崎克罗诺肠杆菌ATCC 29004生物被膜形成的影响。研究首先通过活菌计数法评估不同条件下阪崎克罗诺肠杆菌粘附及生物被膜中的活菌量反映菌体粘附及成膜能力;随后借助场发射扫描电镜观测不同因素对菌体生物被膜微观结构的影响;最后分析不同条件下形成的生物被膜对三种季胺类消毒剂的抗性。结果表明:25 ℃和37 ℃下阪崎克罗诺肠杆菌的粘附菌量和生物被膜形成量高于12 ℃,菌体在胰蛋白胨大豆肉汤中的生物被膜形成量高于在婴幼儿乳粉肉汤中的形成量,37 ℃时硅胶材料表面粘附菌量高于不锈钢和玻璃材料,其他条件下菌体在三种材料表面粘附和生物被膜形成量无明显差异;阪崎克罗诺肠杆菌在25 ℃和37 ℃形成了致密且立体的生物被膜结构,12 ℃生物被膜为单层结构,三种材料表面生物被膜结构呈现出明显差异;阪崎克罗诺肠杆菌生物被膜对消毒剂的抗性取决于菌体的生长温度、接触材料及消毒剂的种类。综上所述,生长温度、培养基质和接触材料对阪崎克罗诺肠杆菌生物被膜形成均有影响,调节生长条件和选择接触材料对于控制阪崎克罗诺肠杆菌生物被膜的形成具有重要意义。
[Key word]
[Abstract]
The present study investigated biofilm formation of C. sakazakii ATCC 29004 at various growth temperatures (12 ℃, 25 ℃ and 37 ℃), incubation medium (tryptone soya broth and infant formula broth) and surface material (stainless steel, glass and silicone). Firstly, attachment and biofilm formation were evaluated by viable counting method. The micro-structure of biofilm formed by C. sakazakii were confirmed by field emission scanning electron microscope. In addition, the effects of growth temperature and surface type on the biofilm resistance to disinfectants were also determined. The results showed that higher temperatures (25 ℃ and 37 ℃) induced stronger attachment and biofilm formation than 12 ℃, the bacterial biofilm formed better in tryptone soy broth than in infant formula broth. The silicone surface had significantly stronger attachment than stainless steel and glass at 37 ℃, but there was no differences in attachment and biofilm formation on different materials at other conditions. C. sakazakii formed flat and thin biofilms when growing at 12 ℃, but C. sakazakii formed compact and thick biofilms at 25 ℃ and 37 ℃. The biofilm structure were different at three different surface materials. The biofilm resistance to disinfectants is affected by growth temperature, incubation medium and surface type. The results indicated that the growth temperature, incubation medium and surface material were important to control biofilm formed by C. sakazakii.
[中图分类号]
[基金项目]
中央高校基本科研业务费专项资金项目(2452017228);中国博士后科学基金项目(2017M623256)