采用简单的滴涂法制备 XC-72 导电碳黑修饰玻碳电极（XC-72/GCE），研究芦丁和芹菜总黄酮在该修饰电极上的电化学 行为及其测定方法。应用循环伏安法（CV）优化了电化学反应条件，包括富集时间、扫速和缓冲溶液的 pH 值；在最佳实验条件下， 应用差分脉冲伏安法（DPV）建立了芦丁标准工作曲线并将其用于芹菜总黄酮含量的测定。实验结果表明，XC-72/GCE 电极对芦丁 的氧化具有良好的电催化活性，其氧化反应为单电子单质子过程；电子传递系数为 0.51。芦丁的 DPV 氧化峰电流（Ip）与其浓度在 4.1×10-8~1.3×10-5 mol/L 范围内呈良好的线性关系，相关系数 r=0.9985，检出限（S/N=3）为 4.1×10-14 mol/L。该修饰电极具有制备简 单，响应快、重复性、稳定性好、抗干扰能力强等优点，可作为一种高灵敏度的电化学传感器应用于芹菜总黄酮含量的测定。此外， 本研究还可为其他蔬菜总黄酮含量检测提供参考。

A glassy carbon electrode modified with XC-72 conductive carbon black was prepared by a simple drop coating method to study the electrochemical behavior and determination of rutin and total flavonoids in celery. Cyclic voltammetry (CV) was used to optimize the electrochemical reaction conditions, including accumulation time, scan rate and pH of the buffer solution. Under the optimal conditions, the standard curve of rutin was established using differential pulse voltammetry (DPV) method for the determination of total flavonoids in celery. The results showed that the XC-72/GCE electrode had good electrocatalytic activity for the oxidation of rutin, and the electrode reaction was a process of one-electron and one-proton transfer with a electron transfer coefficient (α) 0.51. The DPV oxidation peak current (Ip) of rutin had a good linear relationship with its concentration in the range of 4.1×10-8 ~ 1.3×10-5 mol/L with a correlation coefficient of 0.9985 and a detection limit of 4.1×10-14 mol/L (S/N=3). The modified electrode had the advantages of simple preparation, fast response, repeatability, good stability and strong anti-interference capability, which could be used as a high sensitive electrochemical sensor for the determination of total flavonoids in celery. In addition, this research can also provide reference for the determination of total flavonoids in other vegetables.

吉林省大学生创新创业训练计划项目（2016B316、201743）；国家级大学生创新创业训练计划项目（201613706017、201715）；吉林省中医药科技项目（2017102）；吉林省卫生计生科技能力提升计划项目（2016Q054、2017ZC034）；吉林市青年科技工作者创新创业大奖赛获奖项目（201750252）