山葡萄中大量的双糖苷花色苷严重影响山葡萄酒品质，而5GT是合成双糖苷花色苷的关键酶。研究不同葡萄品种间5GT等位基因的差异，为抑制双糖苷花色苷的合成奠定基础，对提高山葡萄酒的品质有重要意义。本研究克隆了‘赤霞珠’、‘左山一’、‘哈桑’和‘左红一’中的5GT等位基因，并用软件对其进行了序列分析和生物信息学分析，共获得了7个5GT等位基因，均位于葡萄9号染色体上，分别编码297~464个氨基酸。序列分析结果表明，4个5GT等位基因由于基因突变可能丧失了5GT功能；7个5GT等位基因均没有信号肽，属于GT1家族中的5GT亚家族。不同葡萄品种中的5GT等位基因存在一定差异，推测这7个5GT等位基因中可能只有3个5GT可以合成双糖苷花色苷。

A large number of diglucosidic anthocyanins in Vitis amurensis seriously affect the quality of wines, and 5GT is the key enzyme for the synthesis of diglycoside anthocyanins. Therefore, studying the differences of 5GT alleles among different grape varieties lay the foundation for inhibiting the synthesis of diglucosidic anthocyanins, which is of great significance for improving the quality of wines made from Vitis amurensis grapes. The 5GT alleles in ‘Cabernet Sauvignon’, ‘Zuoshanyi’, ‘Hasang’ and ‘Zuohongyi’ were cloned and analyzed in this study by sequence analysis and bioinformatics analysis, and seven 5GT alleles were all on chromosome 9 and encoded 297~464 amino acids. The results of sequence analysis showed that four 5GT alleles might lose the function of 5GT due to the gene mutation, seven5GT alleles had no signal peptide and belongs to the 5GT subfamily of GT1. There were some differences in 5GT alleles among different grape varieties. from which we could speculate that only three alleles could synthesize diglucosidic anthocyanins.

国家自然科学基金青年科学基金项目（3101828）；黑龙江省自然科学基金青年科学基金项目（QC2017024）；黑龙江省教育厅科研项目（12541579）；大庆市指导性科技计划项目（zd-2016-108）；黑龙江八一农垦大学引进人才科研启动计划资助项目（XYB2013-16）