[关键词]
[摘要]
本论文研究植物乳杆菌(Lactobacillus plantarum dy-1)发酵大麦提取物(LFBE)及其主要活性组分对秀丽隐杆线虫脂肪沉积的影响。在建立秀丽线虫肥胖模型的基础上,设计不同浓度提取物及主要活性组分作用于肥胖模型,通过油红O染色法分析秀丽线虫体内脂肪含量变化。结果表明,与模型组相比,LFBE能显著降低秀丽线虫脂肪沉积量,其中浓度为1600 μg/mL时,脂肪含量降低了26%,其模型组体脂含量趋于正常组,而未发酵大麦提取物(RBE)则未见此效果;从LFBE中分离出的酚类化合物,总蛋白质,总糖均具有不同程度的减少脂肪沉积的作用,浓度为16 μg/mL的酚类化合物、480 μg/mL的蛋白质、480 μg/mL的总糖,分别能使秀丽线虫脂肪沉积量下降39%、26%及12%。总之,LFBE能显著抑制秀丽线虫体内脂肪的积累,且其中酚类化合物降低脂肪沉积的效果尤为显著。
[Key word]
[Abstract]
The effects of Lactobacillus plantarum dy-1 fermented barley extracts (LFBE) and its major bioactive components on the fat deposition of Caenorhabditis elegans (C. elegans) were investigated in this study. Firstly, the obesity model of C.elegans was established, and then they were fed with different concentrations of extracts and main bioactive components, and the changes of fat content in nematode were analyzed by oil red O staining. The results showed that LFBE could significantly reduce the fat deposition of C. elegans compared with the model group, , which decreased by 26% at the concentration of 1600 μg/mL, and the body fat content of the model group tended to be normal, while the unfermented barley (RBE) showed no the same effect. Total protein, polysaccharide and polyphenol compounds isolated from LFBE had different effects on reducing fat deposition. The fat deposition of C. elegans decreased by 39%, 26% and 12% at the phenolic compounds, protein and polysaccharide concentrations of 16 μg/mL, 480 μg/mL and 480 μg/mL, respectively. In conclusion, LFBE could significantly inhibit the accumulation of fat in C. elegans and the phenolic compounds were particularly effective in reducing fat deposition.
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[基金项目]
国家自然科学基金青年项目(31701605);镇江市重点研发计划(现代农业)(NY2017009)