本文研究了经培养基优化所得牛樟芝微粉及其功能饮料对乙醇诱导的急性酒精肝损伤小鼠和链脲佐菌素（STZ）诱导的糖尿病小鼠的预防保护作用。结果表明：该牛樟芝微粉能有效降低急性酒精肝损伤小鼠血清中ALT、AST和MDA水平（p<0.01），显著提高SOD、CAT和GSH-Px活性（p<0.05或p<0.01），发挥抗氧化作用而减轻肝脏损伤；同时该牛樟芝微粉及其功能饮料均能明显降低糖尿病小鼠的空腹血糖值、糖耐量以及血清中TG、TC和LDL含量，提高HDL含量（p<0.05或p<0.01）。进一步分析能量代谢信号通路，发现该牛樟芝微粉及其功能饮料可能通过下调肝脏中AMPKα和AKT磷酸化水平，进而发挥降血糖和改善血脂代谢的功效。因此，该经培养基优化所得牛樟芝微粉对小鼠酒精性肝损伤和糖尿病均有明显的预防保护作用，这可能是通过抗氧化应激、调控机体能量代谢相关因子的表达来实现，具备开发为解酒护肝降血糖功能饮料的前景。

The hepatoprotective and hypoglycemic effects of Antrodia camphorata powder obtained by medium optimization and its functional beverage on alcohol-induced acute liver injury mice and streptozotocin (STZ)-induced diabetic mice were investigated in this study. Antrodia camphorata powder could significantly reduce the serum levels of ALT, AST, and MDA (p<0.01), and significantly increase the activities of SOD, CAT, and GSH-Px to alleviate the liver injury (p<0.05 or p<0.01). Meanwhile, both the pretreatment of Antrodia camphorata powder and its functional beverage could significantly decrease the levels of fasting glucose, glucose tolerance, TG, TC, and LDL significantly, and could increase the level of HDL significantly (p<0.05 or p<0.01). Through investigating the energy metabolic pathways, Antrodia camphorata powder and its functional beverage could improve the glucose and lipid metabolism of diabetic by down-regulating the phosphorylation levels of AMPKα and AKT in the liver. Therefore, Antrodia camphorata powder had obvious functions to protect against alcohol-induced acute liver injury and STZ-induced hyperglycemia in mice, which might be achieved by anti-oxidative stress and the regulation of energy metabolism related factors, and could be developed as a functional beverage for relieving alcoholism, protecting liver, and reducing blood glucose in the future.

厦门市科技计划项目（3502Z20161229）