喹诺酮类药物作为广谱抗菌药被广泛应用在畜牧养殖业，但如果用药过程使用不规范就会导致兽药残留于动物源性食品中，对于消费者的食用安全造成潜在的隐患。因此，建立一种检测喹诺酮类药物快速、简便的检测方法成为必然。本实验采用蓝色乳胶微球作为标记材料，标记诺氟沙星单克隆抗体，优化建立蓝色乳胶微球标记的喹诺酮类免疫层析试纸条方法，为快速大通量检测食品中兽药残留提供理论支持。通过优化试纸条的各项条件，方法检出限为1 μg/L。选取可能存在兽药残留的猪肉、虾、鸡肉样品，进行添加回收实验，样品检出限均为10 μg/kg。建立的方法可检测环丙沙星，恩氟沙星，氟罗沙星，麻保沙星，依诺沙星，罗氟哌酸6种喹诺酮类兽药残留，试纸条稳定性良好，有效期至少为三个月。

Quinolones were widely used in livestock breeding industry as the broad-spectrum antimicrobial drugs. However, veterinary drugs would remain in animal-derived foods if the use of quinolones was not standardized, causing the potential risks to consumers’ safety.. Therefore， it was inevitable to establish a rapid and simple detection method for quinolones. In this study, blue latex microspheres were used as a marker material to mark norfloxacin monoclonal, and the established method of blue latex microsphere-labeled quinolone immunochromatographic test strips was optimized to provide theoretical support for rapid detection of veterinary drug residues in foods. The detection limit of this method was 1 μg/L under optimized the conditions, and samples of pork, shrimp and chicken that might have veterinary drug residues were selected for the recovery test, the detection limits of the samples were all 10 μg/kg. The established method could detect 6 quinolone residues, including ciprofloxacin, enrofloxacin, fleroxacin, marbofloxacin, enoxacin and norfloxacin, and the test trips have good stability with an effective period of at least three months.

国家自然科学基金资助项目（31301462）