以虎掌菌为萃取原料，采用超临界CO2萃取技术进行虎掌菌精油的提取，在单因素试验的基础上，设定CO2流量为25 kg/h，原料粉碎粒度为40目，一次性投料量为800 g，通过响应曲面法（Response surface methodology，RSM）建立数学模型，确定最优工艺条件：选择萃取压力、温度、时间为影响因子，以虎掌菌精油萃取得率为响应值进行响应面分析，结果表明在萃取压力33 MPa，萃取温度55 ℃，萃取时间145 min时虎掌菌精油萃取得率最大为4.18±0.46%，与理论值（4.23%）相接近。此外，采用气相色谱-质谱联用法（GC-MS）对虎掌菌精油的挥发性成分进行分析，通过人工解析结合标准图谱数据库（NIST）检索定性确认，共检测出77种化学成分，采用峰面积归一化法计算各化合物的相对百分含量，化合物含量占总面积的68.868%，其中主要成分为酸类28.09%，醛类18.77%，含氧杂环5.13%，烃类4.90%，酚类4.37%，醇类4.02%和酮类3.61%等，含量较丰富的有有2-甲基-己酸（19.46±0.81%），苯甲醛（6.86±0.17%）。

Using Sarcodon aspratus as the raw material, supercritical carbon dioxide (SC-CO2) extraction method was employed to extract the essential oil in Sarcodon aspratus. Based on the single factor experiment, the CO2 flow rate was set at 25 kg/h, the particle sizes-mash was 40 meshes and material mass was 800 g. The mathematical model was established by the response surface methodology (RSM) to determine the optimal process conditions. The extraction pressure, temperature and time were used as the Box-Behnken design variables. The response rate of the extract of Sarcodon aspratus oil was the response value. The results showed that the extraction yield of Sarcodon aspratus oil was 4.18 ± 0.46% when the extraction pressure was 33 MPa, extraction temperature was 55℃ and extraction time was 145 min, which was close to the theoretical value (4.23%). Moreover, gas chromatography coupled to mass spectrometry (GC-MS) analysis was carried out to analyze the volatile components of the essential oil of Sarcodon aspratus. The qualitative analysis was confirmed by manual analysis and the National Institute of Standards and Technology (NIST) library. A total of 77 kinds of volatile compounds and the peak were detected. The area normalized method was used to calculate the relative percentage of each compound. The compound content accounted for 68.868% of the total area, of which the main components were acids (28.09%), aldehydes (18.77%), oxygen heterocyclic ring (5.13%), hydrocarbons (4.90%), phenols (4.37%), alcohol (4.02%), ketones (3.61%), etc. 2-methyl-hexanoic acid (19.46 ± 0.81%) and benzaldehyde (6.86 ± 0.17%) were comparatively rich in the essential oil of Sarcodon aspratus.

云南省科技计划项目重大科技专项（2016ZA008）；云南省科技计划项目省院省校科技合作专项（2013IB010）；昆明市畜产品加工与质量控制重点实验室（2015-1-A-00563）