本文探究了魔芋中主要成分葡甘聚糖（Konjac glucomannan，简称KGM）对小鼠肠道的生理结构及蠕动功能的影响。模拟人肠道动力不足之症，采用硫糖铝建模的青年小鼠（4周龄）和老年小鼠（60周龄）来探究不同剂量（低剂量：0.18 g/kg及高剂量：0.90 g/kg）葡甘聚糖对不同年龄小鼠肠道运动功能的影响。在给药后观察首次排便时间、测量排便量、含水量及碳末推进率。结果表明，与空白对照组相比，高剂量葡甘聚糖作用于硫糖铝处理的模型小鼠均能使青、老年小鼠首次排便时间明显延长，排便量下降的现象。H&E染色结果表明高剂量葡甘聚糖处理后的老年小鼠中，易见结肠炎性细胞聚集以及小肠完整结构破坏。因此，使用剂量偏大可能为大多数肠道动力不足的老年人过量食用葡甘聚糖后排便困难的重要原因之一。

Effects of Konjac glucomannan (KGM) on theintestinal physiological structure and peristaltic function in mice were investigated in this study. Modeling human intestinal motility disorders, using sucralfate-modeled young mice (4 weeks old) and old mice (60 weeks old) to explore different doses (low dose:0.18 g/kg and high dose:0.90 g/kg) of KGM on intestinal motility in mice with different ages. After the administration, the defecation time, stool weight, fecal water content, carbon propulsion rates were determined. The results showed that compared with the control group, the high dosage of KGM treatment on sucralfate-treated young and aged mice could significantly prolong the first defecation time and decrease the stool weight. The results of H&E staining showed that the structure integrity of intestinal tissues were destroyed and colonic inflammatory cell aggregation increased in the aged mice treated with high dose of KGM. high dose uptakes of KGM could result in insufficient intestinal motility and the difficulty of defecation in aged people.

国家自然科学基金资助项目（81573887）