为探究百合花醇提物花青素对CCl4所致的大鼠肝纤维化的预防、改善作用及可能机制。将32只大鼠随机分为对照组（Sham）、肝纤维化模型组（M）、百合花花青素低剂量组（LFF 0.5 g/mL）和高剂量组（LFF 1.0 g/mL）。M组、LFF组大鼠背部皮下注射50% CCl4造模，每周2次，连续8周。LFF组大鼠给予不同剂量的百合花花青素提取物灌胃，Sham组和M组给予等体积的生理盐水，每天1次。8周后处理动物，采集血清检测ALT、AST；肝组织匀浆测定SOD、CAT、GSH-Px活力和MDA含量；Western-blot测定肝组织TGF-β1和α-SMA的表达。实验结果表明，与模型M组比较，百合花花青素能明显降低大鼠血清中ALT、AST水平；使肝组织抗氧化酶活力SOD、CAT、GSH-Px显著上升，脂质过氧化物MDA生成减少，进而诱导肝组织纤维化相关物质TGF-β1、α-SMA表达下调。百合花花青素具有良好的抗氧化活性，改善大鼠肝损伤，并逆转CCl4诱导的肝纤维化，其机制可能与抗脂质过氧化物损伤及下调细胞外纤维组织增生有关。

In order to explore the prevention, improvement and possible mechanism of alcohol extract anthocyanin from Lilium lancifolium on the liver fibrosis induced by CCl4 in rats, thirty-two rats were randomly divided into control group (Sham), liver fibrosis model group (M), low dose lancifolium anthocyanin group (LFF 0.5 g/mL) and high dose lancifolium anthocyanin group (LFF 1.0 g/mL). Rats in M group and LFF group were subcutaneously injected with 50% CCl4 on the back, twice a week for 8 weeks. The rats in M group and LFF group were subcutaneously injected with 50% CCl4 on the back twice a week for 8 weeks. The rats in LFF group were given different doses of alcohol extract anthocyanin from Lilium lancifolium. Sham group and M group were given the same volume of physiological saline once a day. After eight weeks, the animals were sacrificed and serum was collected for detecting alanine amiotransferase (ALT) and aspartate aminotransferase (AST). The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-px) and the content of malondialdehyde (MDA) in the liver homogenate were measured. Western blot was employed to examine the expression of transforming growth factor-β 1 (TGF-β1) and alpha-smooth muscle actin (α-SMA) in the liver tissue. The results showed that compared with M group, Lilium lancifolium anthocyanins could obviously reduce the ALT and AST levels in rat serum, and significantly increase the activities of SOD, CAT and GSH-Px in liver tissue, decrease the lipid peroxideMDA, and then down-regulate the expression of liver fibrosis related materials such as TGF-β1 and α- SMA. Lilium lancifolium anthocyanins had good antioxidant activity, which could significantly reverse the liver fibrosis induced by CCl4. The mechanism might be related to resist the damage of lipid peroxide and reduce the proliferation of extracellular fibrous tissue.

湖北科技学院校级基金项目（2016-18XZY03）