在工业生产中，酿酒酵母进行高温发酵具有发酵速度快、发酵周期短、发酵原料黏度低、生产成本少并且更适用于同步糖化发酵工艺等优点，因此，选育耐高温的酿酒酵母菌株在工业应用方面具有重要意义。本研究以从工业菌种中分离出来的酿酒酵母AY12作为出发菌株，对其进行常压室温等离子体诱变，在37 ℃下进行初筛，得到150株单菌落长势较好的菌株。将其进行产孢、杂交以实现基因组重排，在37 ℃下进行复筛，得到137株生长性能良好的菌株。在35 ℃玉米水解液中发酵，进行二次复筛，从而获得具有良好发酵性能的14株菌株。最后，进行35 ℃同步糖化发酵，并测定每菌株的乙醇产量和残糖含量。，最终筛选出7株具有良好耐高温发酵性能的菌株，其中发酵性能最好的菌株为L-38，其同步糖化发酵完成后，乙醇产量为16.20%（V/V），较出发菌株AY12提升了8.00%，残糖含量为35.50 g/L，较AY12降低了32.38%。

Saccharomyces cerevisiae has the advantages of fast fermentation speed, short fermentation cycle, low viscosity of fermentation raw materials and low production cost during the high temperature fermentation in industrial production, which is more suitable for simultaneous saccharification fermentation technique. Therefore, S. cerevisiae strain with high temperature tolerance is of great significance in the industrial application. In this study, AY12 was used as the starting strain, which was isolated from industrial strains. The strain was mutated by atmospheric and screened at 37 ℃. Then, 150 strains with good colony growth were obtained. By the way, the stains were carried out to achieve genome shuffling by sporulation and hybridization, and 137 strains with good growth performance were obtained by re-screening at 37℃. After fermentation in the corn hydrolysate at 35 ℃, 14 strains were obtained with good fermentation performance. The strains were rescreened by the simultaneous saccharification fermentation at 35 ℃, and the ethanol yield and residual sugar content of each strain were determined. Finally, 7 strains were screened with good tolerance to high temperature fermentation. L-38 was screened as the best fermentation performance strain after the simultaneous saccharification fermentation. Compared with the starting strain AY12, the ethanol yield of L-38 was 16.20% (V/V), which increased by 8.00%., and the residual sugar content was 35.50 g/L, which was 32.38% lower than that by AY12.

国家重点研发项目（2016YFD0400505）；“十二五”农村领域国家科技计划（2013AA102108）