[关键词]
[摘要]
研究人工接种炭疽病菌对采后香蕉果实贮藏品质的影响。分析测定了接种炭疽病菌后“宝岛蕉”和“东莞大蕉”两个品种香蕉果实的硬度、叶绿素含量、可溶性固形物含量、还原糖含量、可滴定酸含量、呼吸强度、病情指数及抗病相关酶POD、PPO活性等指标的变化。结果显示,接种炭疽病菌的香蕉果实的硬度迅速下降,贮藏4 d后显著低于未接种炭疽病菌组,香蕉果皮叶绿素含量的分解速率显著增加,加速了香蕉褪青和转黄;接种炭疽病菌促进了果实中淀粉等多糖物质不断地向可溶性糖的转化,可溶性固形物、还原糖含量明显增加,对“宝岛蕉”的影响更为明显。炭疽病菌侵染促进了香蕉果实中可滴定酸含量的升高,同时加快果实的呼吸和感病速率;接种炭疽病菌后,抗病相关酶POD、PPO活性显著升高。结果表明炭疽病菌的侵染加速了两种香蕉的成熟、软化、衰老及腐烂。
[Key word]
[Abstract]
The effects of artificial inoculation with Colletotrichum musae on the postharvest quality of banana fruit were determined. Two banana cultivars (Musa acuminata L. AAA Cavendish ‘Formosana’ and Musa spp. ABB) were inoculated with C. musae, and changes in the hardness, chlorophyll content, total soluble solid content, reducing sugar content, titratable acid content, respiration intensity, and activities of enzymes related to disease resistance (polyphenol oxidase and peroxidase) were analyzed. The results showed that fruit hardness decreased rapidly after inoculation with C. musae, and was significantly lower than that of the control after four days of storage. C. musae infection led to a significant increase in the rate of chlorophyll decomposition in banana pericarp and the rate of the green-brown color change in banana fruit. Furthermore, C. musae infection promoted the continuous transformation of polysaccharides (e.g., starch) in fruit into soluble sugars, resulting in a significant increase in soluble solid and reducing sugar content; these changes were more pronounced in the Musa spp. ABB cultivar. C. musae infection accelerated the increase in titratable acid content during storage, enhanced the respiration rate and the rate of infection, and significantly increased the activities of polyphenol oxidase and peroxidase in banana fruit. These results indicate that infection with C. musae can accelerate the maturation, softening, senescence, and decay of banana fruit.
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[基金项目]
国家自然科学基金项目(31160407、31560467、31660589);国家公益性行业(农业)科研专项(201303073);“八桂学者”工程专项经费([2016]21);国家中组部“万人计划”青年拔尖人才项目(组厅字[2015]48号);广西自然科学基金项目(2014GXNSFAA118110、2014GXNSFDA118013、2015GXNSFBA139102);广西农业重点科技计划项目(201527);广西科学研究与技术开发计划项目(桂科合15104001-23、桂科合1347004-18、桂科AD16380015);留学人员科技活动项目择优资助项目(人社厅函[2015]192号);南宁市科学研究与技术开发计划(20142305);广西农业科学院基本科研业务费项目(桂农科2015YT86、桂农科2015JM14、桂农科2014YQ05、桂农科2014JQ04、桂农科2015JZ75、农成转2016018、2017NZ13)