本文对提取得到的高粱糠不同存在形态酚类物质进行AB-8大孔树脂纯化，之后进行高效液相色谱分析及抗AAPH氧化溶血效果的测定。经AB-8大孔树脂纯化后，总酚含量依次为游离酚、束缚型酯型酚、束缚型苷型酚、可溶性苷型酚、可溶性酯型酚。而HPLC的分析结果显示，所检测到的18种酚类化合物的总量顺序依次是：束缚型酯型酚>游离酚>束缚型苷型酚>可溶性酯型酚>可溶性苷型酚。200 μg/mL的游离型、束缚型和可溶型酯型酚可使AAPH溶血率降至约10%，接近阴性对照组，而可溶性苷型酚仍为28.84%。五类酚类物质的预处理使得AAPH损伤的人血红细胞内的MDA含量减少，GSH/GSSG升高，使红细胞恢复到正常状态；SOD、GSH-Px和CAT酶活力较弱的红细胞也受到了保护。综上，经AB-8大孔树脂纯化后的高粱糠不同存在形态酚类物质可以保护人血红细胞免受AAPH的损伤，其细胞内抗氧化活性与总酚含量和酚类化合物的组成有联系。

Different types of phenols extracted from sorghum bran were purified on the AB-8 macroporous resin and then analyzed by high performance liquid chromatography (HPLC). The inhibitory effects of the phenols on human-erythrocyte hemolysis caused by 2,20-azobis-(2-amidinopropane) dihydrochloride (AAPH) were studied. The results showed that after purification on the AB-8 macroporous resin, phenol concentrations were in the following order: free phenols > insoluble bound esterified phenols > insoluble bound glycosidic phenols > soluble glycosidic phenols > soluble esterified phenols. According to the results of HPLC, the concentrations of 18 phenolic compounds were in the following order: insoluble bound esterified phenols > free phenols > insoluble bound glycosidic phenols > soluble esterified phenols > soluble glycosidic phenols. After the treatment with 200 μg/mL insoluble bound esterified, free, insoluble bound glycosidic, or soluble esterified phenols, the rate of AAPH-induced hemolysis of erythrocytes decreased to ~10%, which was close to that of the negative control group; the AAPH hemolysis rate of erythrocytes in the soluble glycosidic phenolic (200 μg/mL) treatment group was still 28.84%. Pretreatments with five different types of phenols effectively reduced the concentration of malondialdehyde (MDA), increased the glutathione/glutathione disulfide (GSH/GSSG) ratio in the AAPH-treated erythrocytes, and restored the erythrocytes to the normal state. The erythrocytes with relatively low superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities were also protected. In conclusion, the different types of phenols from sorghum bran after purification on the AB-8 macroporous resin may protect human erythrocytes from the damage caused by AAPH-generated free radicals, and their intracellular antioxidant activity is related to their total phenolic content and composition of a mixture of phenolic compounds.

中央高校基本科研业务费专项资助项目（2013ZM0065）；广东省天然产物绿色加工与产品安全重点实验室开放基金资助项目（201304）；广东省高等学校科技创新重点项目（2012CXZD0009）；国家自然科学基金项目（31201330）；广州市科技攻关项目（201300000202）