啤酒酵母作为啤酒生产中主要的副产物具有较高的开发利用价值，本文以啤酒酵母为材料，通过自溶以及乙酸乙酯萃取获得除味啤酒酵母提取物，研究了其防紫外线与酪氨酸酶抑制效果、DPPH清除力、吸湿与保湿性等护肤功效。结果表明，经自溶及乙酸乙酯萃取获得的除味啤酒酵母提取物的蛋白质量浓度为4.77 g/L，该提取物对紫外线的平均吸收率为85.08%，对UVA的平均吸收率为64.25%，对UVB的平均吸收率为93.42%，对UVC的平均吸收率为100.00%，对酪氨酸酶的抑制率为97.90%，对DPPH·的清除力为31.12%，吸湿性显著高于甘油，保湿性与甘油效果相当。结果表明，除味酵母提取物可以作为良好的UVB吸收剂以及酪氨酸酶抑制剂，避免紫外线导致的晒伤与皮肤癌等问题，有效清除自由基，具有防晒美白、抗氧化和保湿等多重护肤功效。

Brewer’s yeast, as the main byproduct of the beer industry, has a high value for development and utilization. Here, brewer’s yeast was used as the raw material from which an odorless extract was prepared by autolysis and ethyl acetate extraction. Ultraviolet (UV) protection, tyrosinase inhibitory effect, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability, hygroscopicity, moisture retention, and other skin care benefits of the extract were studied. The results showed that when the protein mass concentration of the odorless extract of brewer’s yeast was 4.77 g/L, the average UV, UVA, UVB, and UVC absorbance, the tyrosinase inhibition rate and DPPH scavenging rate were 85.08%, 64.25%, 93.42%, 100%, 97.90%, and 31.12%, respectively. The hygroscopicity of this extract was significantly higher than that of glycerin, and the moisture-retention capacity of this extract was equivalent to that of glycerin. The results indicate that the odorless yeast extract could serve as a good UVB absorber and tyrosinase inhibitor. It could also prevent sunburn caused by UV absorption, skin cancer, and other associated health problems. It also shows sun screening and whitening effects, antioxidant activity, moisture retention capacity, and other skin care benefits.

广东省自然科学基金项目（2014A030313423）；广东省科技计划项目（2014A010107026）