[关键词]
[摘要]
借助超高效液相色谱联用质谱(UPLC-Triple-TOF-MS/MS)分析法,对不同泌乳期人乳中的磷脂进行定性和相对定量,为中国人乳磷脂组成的研究提供参考。本研究从哈尔滨(黑龙江省)、齐齐哈尔(黑龙江省)和吉林(吉林省)地区采集到汉族人初乳(1~5 d)、过渡乳(6~20 d)和成熟乳(21 d~)共计73份。LipidView 1.2用于检索磷脂分子式,PeakView 2.2及其插件MasterView 1.1用于磷脂定性分析,MultiQuant用于磷脂相对定量分析(基于色谱峰面积)。结果显示,人乳中检出磷脂共计60种。其中,磷脂酰胆碱(PC)17种,磷脂酰乙醇胺(PE)25种,磷脂酰丝氨酸(PS)4种,磷脂酰肌醇(PI)5种,鞘磷脂(SM)9种。磷脂酰胆碱(PC)、磷脂酰乙醇胺(PE)、鞘磷脂(SM)、磷脂酰丝氨酸(PS)和磷脂酰肌醇(PI)在总磷脂中的相对含量分别为38.12%、26.97%、29.54%、4.43%和0.94%,各种磷脂的相对含量随泌乳期变化表现出不同的变化趋势,但大多数磷脂的相对含量差异并不显著。
[Key word]
[Abstract]
Ultra-performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight tandem mass spectrometry (UPLC-Triple-TOF-MS/MS) was used for the qualitative and relative quantitative analysis of phospholipids in human milk during different lactation periods, and to provide a reference for the analysis of phospholipid composition of milk from Chinese women. A total of 73 human milk samples including colostrum (1~5 d), transitional milk (6~20 d), and mature milk (after 21 d) were collected from Harbin (Heilongjiang province), Qiqihar (Heilongjiang province), and Jilin (Jilin province). LipidView 1.2, PeakView 2.2 with MasterView? 1.1 plugin, and MultiQuant? were used for the molecular formula search, qualitative analysis, and relative quantitative analysis of phospholipids, respectively. Sixty species of phospholipids were identified in the milk samples, and the numbers of phosphatidylcholines (PCs), phosphatidylethanolamines (PEs), phosphatidylserines (PSs), phosphatidylinositols (PIs), and sphingomyelins (SMs) were 17, 25, 4, 5, and 9, respectively. The relative contents of PCs, PEs, SMs, PSs, and PIs in the total phospholipids were 38.12%, 26.97%, 29.54%, 4.43%, and 0.94%, respectively. In addition, the relative contents of the various phospholipids varied with the lactation period, although the changes in the relative contents of most phospholipids were not significant.
[中图分类号]
[基金项目]
特色农产品辐照保鲜技术服务企业行动计划(黔科合平台人才[2016]5712号);贵州省特色农产品辐照保鲜与加工工程技术研究中心(2016GZ67305)