本文研究了红光对紫色红曲霉M9生长及色素和桔霉素产量的影响。采用高效液相色谱法对六种红曲色素，包括两种红色素：红斑红曲胺（rubropunctamine，RUM）和红曲玉红胺（monascorubramine，MOM）；两种黄色素：红曲素（monascin，MS）和红曲黄素（ankaflavin，AK）；两种橙色素：红斑红曲素（rubropunctatin，RUN）和红曲玉红素（monascorubrin，MON）以及桔霉素的产量进行测定。结果表明：持续红光照射促进了紫色红曲霉M9菌丝生长、色素合成积累以及孢子形成，尤其对于闭囊壳的产生有更显著促进作用。5种不同的红光照射条件促进了RUM、MOM、MS和AK的产生，抑制了RUN、MON和桔霉素的产生。在光照强度300 lux，光照时间30 min/d，光照节律12 h的最佳光照条件下，RUM、MOM的产量分别提高了53.8%和75.2%；MS和AK的产量分别提高了42.2%和59.4%；RUN和MON的产量分别降低了42.6%和54.5%；桔霉素的产量降低了42.5%。

Red light was used to illuminate Monascus purpureus M9 to evaluate its effects on growth, pigments, and citrinin production. The contents of citrinin and six intracellular pigments, rubropunctamine (RUM), monascorubramine (MOM), monascin (MS), ankaflavin (AK), rubropunctatin (RUN), and monascorubrin (MON), from Monascus purpureus M9 were measured using high-performance liquid chromatography (HPLC). The results indicated that continuous illumination with red light promoted mycelial growth, the synthesis and accumulation of pigments, and spore formation in Monascus purpureus M9, especially the production of cleistothecia. Furthermore, five illumination conditions stimulated the production of RUM, MOM, MS, and AK, but inhibited the yields of RUN, MON, and citrinin. Under the optimal red light illumination condition (light intensity: 300 lux, illumination time: 30 min/d, and lighting regime: 12 h), the yields of RUM, MOM, MS, and AK were increased by 53.8%, 75.2%, 42.2%, and 59.4%, respectively, and those of RUN, MON, and citrinin were decreased by 42.6%, 54.5%, and 42.5%, respectively.

国家自然科学基金资助项目（31330059、31571820）