谷物膳食对人体健康以及癌症、心脏病和糖尿病等的预防具有重大意义，而晚期糖基化终产物（AGEs）会诱导糖尿病等并发症的产生，因此本试验选择从麦麸中提取得到的麦麸酶解物和阿魏酰低聚糖作为抑制剂对牛血清蛋白-葡萄糖模拟体系中AGEs进行抑制研究。选用阿魏酸、低聚木糖以及不溶性膳食纤维作为对照组，以AGEs的荧光产物和非荧光产物（羧甲基赖氨酸）的生成量来评价抑制效果。结果显示，从麦麸中提取得到的阿魏酰低聚糖中的总多酚和总黄酮的量是麦麸酶解物中含量的5.36和2.67倍，且阿魏酰低聚糖和麦麸酶解物对AGEs中荧光产物的抑制率分别为14.49%和5.75%，对羧甲基赖氨酸的抑制率分别为18.16%和16.59%，抑制率的不同可能与其总多酚和黄酮的含量不同有关。阿魏酸、低聚木糖以及不溶性膳食纤维组对AGEs中荧光产物和CML基本没有抑制效果。通过检测模拟体系反应前后还原糖和赖氨酸的含量可知，阿魏酰低聚糖和麦麸酶解物可能是通过减少蛋白中可用赖氨酸的反应量从而抑制糖基化反应进程。

Cereal foods are important for human health and for the control and prevention of cancer, heart disease, and diabetes, while advanced glycation end products (AGEs) can induce the occurrence of diabetes and other complications. Therefore, feruloyl oligosaccharides and wheat bran hydrolysate were selected as the inhibitors in this experiment to study their inhibitory effects on the formation of AGEs in bovine serum albumin-glucose system. Ferulic acid, xylooligosaccharides, and insoluble dietary fiber were used as controls, and the fluorescent and non-fluorescent products (Nε-carboxymethyllysine and CML) of AGEs were used to evaluate the effects of the inhibitors. The results indicated that the contents of total polyphenol and total flavonoids in feruloyl oligosaccharides were 5.36 and 2.67 times as much as those in wheat bran hydrolysate, respectively. The inhibition rates of feruloyl oligosaccharides and wheat bran hydrolysates on the fluorescent products were 14.49% and 5.75%, respectively, and their inhibition rates on the non-fluorescent product CML were 18.16% and 16.59%, respectively. The different inhibition rates might be related to the varying levels of total polyphenols and flavonoids. Ferulic acid, xylooligosaccharides, and insoluble dietary fiber groups showed no inhibitory effect on the production of fluorescent and non-fluorescent products. As evident from the amounts of reducing sugar and lysine in the simulation system before and after the reaction, feruloyl oligosaccharides and wheat bran hydrolysate may inhibit the glycosylation reaction by reducing the content of available lysine in the reactive proteins.

国家自然科学基金项目（31571940）