本文利用九香虫对慢性锰中毒的雄性SD大鼠生殖系统损伤进行干预，并探讨其作用机制。结果表明，一方面九香虫低、中、高干预组大鼠交配行为能力中插入次数分别是模型组的2.65、2.62和1.65倍；射精次数分别是模型组的2.01、1.94和1.25倍；同时血清睾酮水平较模型组分别提高了1.14、1.22和1.23倍，说明九香虫能够修复慢性锰中毒大鼠生殖功能的损伤；另一方面九香虫各干预组的大鼠睾丸组织形态结构虽明显优于模型组，但睾丸和附睾的脏器系数以及精子数目等生殖能力方面的改善不明显。另外，九香虫低、中、高干预组睾丸组织T-SOD水平分别提高了1.23、1.54和1.60倍，且T-AOC水平也分别提高了1.28、1.54和1.39倍，认为九香虫对慢性锰中毒的雄性SD大鼠生殖系统损伤的保护作用可能与其抗氧化作用有关，为生殖损伤的药物开发提供了选择。

Aspongopus chinensis was used for the intervention of damages caused by chronic manganese exposure to the reproductive system of male Sprague Dawley (SD) rats, and its mechanism of action was explored. The results showed that compared with the model group, the mating ability defined by the numbers of penile insertion, in the low-, medium-, and high-dose Aspongopus chinensis intervention groups was boosted by 2.65, 2.62, and 1.65 times, respectively. The corresponding ejaculation frequencies were increased by 2.01, 1.94, and 1.25 times, respectively, and the corresponding levels of serum testosterone were increased by 1.14, 1.22, and 1.23 times, respectively. The results suggested that Aspongopus chinensis could repair damages caused by chronic manganese poisoning to the reproductive function of rats. Meanwhile, the morphological structures of the testicular tissue of the Aspongopus chinensis intervention groups were obviously improved when compared with those of the model group. However, there was no significant improvement in the organ coefficient of the testis and epididymis, the sperm count, as well as other aspects of reproductive ability. Furthermore, compared with the model group, the total superoxide dismutase (T-SOD) activity of the low-, medium-, and high-dose Aspongopus chinensis intervention groups was increased by 1.23, 1.54, and 1.60 times, respectively, and the corresponding total antioxidant capacities (T-AOCs) were increased by 1.28, 1.54, and 1.39 times, respectively. The results suggested that the protective effect of Aspongopus chinensis on reproductive injury in male SD rats was related to its antioxidant activity. This study provides a basis for the development of drugs to treat reproductive injury.

贵州省教育厅“125计划”重大科技专项（黔教合重大专项字[2014]031）；遵义市科技创新人才团队培养项目（遵市科合[2015]40号）；贵州省中药现代化科技产业研究开发项目（黔科合中药字[2012]5001-1号）；贵州省“西部之光”项目；遵义医学院博士启动基金项目（[2013]69）