本文考察不同极性大孔树脂、离子交换树脂、活性炭及硅胶等吸附介质对红曲菌深层发酵液中水溶性红曲黄色素的吸附分离性能。结果显示非极性大孔树脂DA201-C效果最好，该吸附过程符合Freundlich方程，吸附动力学符合准一级动力学模型，表明吸附不受单层吸附的限制；液膜扩散是吸附主要限速步骤，扩散速率常数为0.16 min-1。通过静态和动态吸附洗脱条件优化，运用SPSS对数据进行分析，发现静态吸附、解吸料液比为1:2，吸附、解吸时间15 min，解吸液100%乙醇，酸性条件解吸效果较好；动态吸附解吸流速为0.25 BV/min条件下，可得到高回收率（96.99%）、脱盐率（99.44%）和脱糖率（92.52%）的水溶性红曲黄色素，经初步扩大实验显示吸附解吸性能稳定。大孔树脂DA201-C分离纯化发酵液中水溶性红曲黄色素操作简单、快速、放大性能好，具有很好的工业化生产应用价值。

The adsorption and isolation capacities of macroporous resins with different polarities, ion exchange resins, activated carbon, and silica gel on water-soluble yellow pigments of submerged Monascus fermentation broths were studied. The results showed that nonpolar macroporous resin DA201-C exhibited the best performance; the adsorption isotherm of DA201-C was well fitted with the Freundlich equation, and the adsorption kinetics followed the pseudo-first-order kinetics model, indicating that adsorption was not restricted to monolayer adsorption. Liquid film diffusion was the major rate-limiting step, and the diffusion rate constant was 0.16 min-1. The purification conditions were optimized by static and dynamic adsorption and desorption experiments, and the results were analyzed using SPSS software. The optimal desorption performance was achieved when the solid material-to-liquid ratio of static adsorption and desorption was 1:2, both the adsorption and desorption times were 15 min, the eluent was 100% ethanol, and the eluent was acidic. When the flow rate of dynamic adsorption and desorption was 0.25 BV/min, the water-soluble Monascus yellow pigments could be obtained with high recovery (96.99%), desalination (99.44%), and desugarization rate (92.52%). The preliminary scale-up experiment showed a stable adsorption and desorption efficiency. In conclusion, the method using DA201-C resin to isolate and purify the water-soluble Monascus yellow pigments in fermentation liquid is easy to operate, has a short cycle and good performance in a scale-up experiment, and shows a great application value in industrial production.

国家自然科学基金项目（31271925）；广东省教育部产学研结合项目（2013B090600015）；广州市科技计划项目（2014J4100192）