探讨不同极性酸浆提取液对体外诱导的非酒精性脂肪肝（NAFLD）细胞的影响及其可能的作用机制。用不同极性有机溶剂对酸浆进行萃取，采用MTT法筛选医用脂肪乳注射液使用浓度、酸浆提取液作用浓度，光学显微镜观察油红O染色情况，全自动生化仪检测甘油三酯（TG）、谷丙转氨酶（ALT）、谷草转氨酶（AST）和谷氨酰转肽酶（GGT）水平。与正常肝细胞株HL7702相比，模型组油红O染色后细胞浆内有大量被染成橘红色的脂滴，细胞内TG、ALT、AST和GGT水平显著升高（p<0.01）。与模型组相比，不同酸浆提取物治疗12 h组细胞浆内橘红色的脂滴数量明显减少，TG、ALT、AST和GGT水平显著降低（p<0.01）。在体外成功建立非酒精性脂肪肝的细胞模型，不同极性酸浆提取液处理能减轻NAFLD细胞中TG蓄积和ALT、AST和GGT的泄漏量。

The aim of this study was to investigate the effects of Physalis alkekengi extracts with different polarities in an in vitro model of induced nonalcoholic fatty liver disease (NAFLD) and identify the possible mechanism. Physalis alkekengi samples were extracted using organic solvents with different polarities. The concentrations of medical fat emulsion for injection and Physalis alkekengi extracts were selected by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Lipid droplets stained with oil red O were observed under a light microscope. The triglyceride (TG), alanine aminotransferase (ALT), aspartate transaminase (AST), and gamma-glutamyl transpeptidase (GGT) levels were determined by an automatic biochemical analyzer. Compared with normal liver cells, HL7702, the model group showed a large quantity of orange-red lipid droplets in the cytoplasm after oil red O staining, and the intracellular TG, ALT, AST, and GGT levels increased significantly (p<0.01). Compared with the model group, the groups treated with different Physalis alkekengi extracts for 12 h exhibited a considerably reduced number of orange-red lipid droplets in the cytoplasm and significantly decreased levels of intracellular TG, ALT, AST, and GGT (p<0.01). An in vitro cell model of nonalcoholic fatty liver disease was successfully established, and treatment with Physalis alkekengi extracts with different polarities could reduce the accumulation of TG and leakage of ALT, AST, and GGT in NAFLD cells.

吉林省教育厅“十二五”科学技术研究项目支助项目（吉教科合字[2013]第360号）；吉林省卫生厅支助项目（2014ZC063）