[关键词]
[摘要]
为揭示茶饼病菌对茶树叶片中游离氨基酸组分及其含量的影响,以3个不同抗性品种茶树的病、健叶为试验材料,利用高效液相色谱法(HPLC)检测不同抗性品种茶树未感病和感病后鲜叶中氨基酸的组分和含量。结果表明:不同抗性品种未感病叶片中游离氨基酸的的种类和含量有差异,茶氨酸含量最高,其次是γ-氨基丁酸、蛋氨酸、胱氨酸;感病后氨基酸总量上升,高抗品种紫娟叶片中游离氨基酸的种类减少,含量下降,仅γ-氨基丁酸含量增加;中抗品种云抗10号叶片中游离氨基酸的种类增加,含量均增加;感病品种佛香1号叶片中游离氨基酸的种类不变,γ-氨基丁酸、茶氨酸、赖氨酸、色氨酸、精氨酸5种游离氨基酸的含量增加,其余氨基酸的含量下降,因而可以以此分析氨基酸与茶树抗病性的关系。本研究结果可为从生理生化角度探讨茶饼病致病机理提供基础数据。
[Key word]
[Abstract]
To explore the effects of Exobasidium vexans Massee (E. vexans) on the amino acid composition and content of Camellia Sinensis (Linn.) var. assamica (Masters) Kitamura, infected and healthy leaves from three Camellia sinensis (L.) Kuntze varieties with different resistance were used as experimental materials, and high-performance liquid chromatography (HPLC) was conducted to measure the amino acid composition and content of the infected and healthy leaves from the tea trees with different resistance. The results showed that there were differences in the concentrations and types of free amino acids among healthy leaves from different resistant varieties. Theanine had the highest concentration, followed by γ-aminobutyric acid, methionine, and cystine. After infection, total amino acid content increased; in the leaves of ZiJuan-the variety with high resistance to E. vexans-the concentrations and diversity of free amino acids decreased, and only the concentration of γ-aminobutyric acid increased; in the leaves of YunKang10-the variety with medium resistance to E. vexans-the concentrations and diversity of free amino acids increased; in the leaves of susceptible variety FoXiang1, the diversity of free amino acids did not change, the concentrations of γ-aminobutyric acid, theanine, lysine, tryptophan, and arginine increased, and concentrations of the other amino acids decreased. Therefore, the above findings can be used to analyze the relation between amino acids and disease resistance of tea trees. The results of this study provide basic data for the research on the pathogenicity of E. vexans from physiological and biochemical points of view.
[中图分类号]
[基金项目]
国家科技支撑计划项目(2007BAD58B01);普洱学院高层次人才科研启动项目(K2015032);农学专业普洱茶实验实习实训基地与加工技术创新服务中心(云高教2015-56号);云南省科技创新强省计划(2014AE014)