卞恒娟,曾本华,王丽,钟青萍,李文霞,魏泓,方祥,廖振林.C57BL/6J伪无菌与SPF小鼠高脂饮食诱导的比较研究[J].,2016,32(11):29-34.
C57BL/6J伪无菌与SPF小鼠高脂饮食诱导的比较研究
Comparative Study of High Fat Diet in Induced C57BL/6J Pseudo Germ-free Mice and Specific-pathogen-free (SPF) Mice
投稿时间:2015-12-24  
DOI:10.13982/j.mfst.1673-9078.2016.11.005
中文关键词:  肠道菌群  肥胖  抗生素  伪无菌动物模型
英文关键词:gut microbiota  obesity  antibiotic cocktails  pseudo germ-free mouse model
作者简介:卞恒娟(1992-),女,硕士研究生,研究方向:食品微生物 通讯作者:廖振林(1968-),男,博士,副教授,研究方向:益生元、益生菌与脂肪代谢;方祥(1971-),男,博士,副教授,研究方向:益生元、益生菌与脂肪代谢
基金项目:国家自然科学基金资助项目(31171673);国家高技术研究发展计划(2014AA022204,2014AA022209)
作者单位
卞恒娟 (1.华南农业大学食品学院,广东广州 510640) 
曾本华 (2.第三军医大学基础部实验动物学教研室,重庆 400038) 
王丽 (1.华南农业大学食品学院,广东广州 510640) 
钟青萍 (1.华南农业大学食品学院,广东广州 510640) 
李文霞 (2.第三军医大学基础部实验动物学教研室,重庆 400038) 
魏泓 (2.第三军医大学基础部实验动物学教研室,重庆 400038) 
方祥 (1.华南农业大学食品学院,广东广州 510640) 
廖振林 (1.华南农业大学食品学院,广东广州 510640) 
AuthorInstitution
BIAN Heng-juan (1.College of Food Sciences, South China Agricultural University, Guangzhou 510640, China) 
ZENG Ben-hua (2.Department of Laboratory Animal Science, College of Basic Medical Sciences, the Third Military Medical University, Chongqing 400038, China) 
WANG Li (1.College of Food Sciences, South China Agricultural University, Guangzhou 510640, China) 
ZHONG Qing-ping (1.College of Food Sciences, South China Agricultural University, Guangzhou 510640, China) 
LI Wen-xia (2.Department of Laboratory Animal Science, College of Basic Medical Sciences, the Third Military Medical University, Chongqing 400038, China) 
WEI Hong (2.Department of Laboratory Animal Science, College of Basic Medical Sciences, the Third Military Medical University, Chongqing 400038, China) 
FANG Xiang (1.College of Food Sciences, South China Agricultural University, Guangzhou 510640, China) 
LIAO Zhen-lin (1.College of Food Sciences, South China Agricultural University, Guangzhou 510640, China) 
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中文摘要:
      肠道菌群与宿主肥胖密切相关,伪无菌小鼠动物模型是研究肠道菌群与肥胖及其他相关疾病关系的有利“工具”。20只8周龄C57BL/6J雄性小鼠给予抗生素混合物饮水,剔除大部分肠道微生物模拟无菌动物。随机分成两组:对照组和高脂组,分别饲喂常规纯化饲料与高脂纯化饲料8周;同时以20只SPF级小鼠作对照。常规方法测定小鼠体重、血清中血糖、血脂指标和胰岛素水平,并用软件测量脂肪细胞大小。结果表明,与高脂组SPF小鼠相比,高脂组伪无菌小鼠的体重、脂肪垫与肾周脂重量显著低于高脂组SPF小鼠;胰岛素水平和脂肪细胞大小也显著低于SPF小鼠。高脂组伪无菌小鼠出现低体重低脂肪的表型,与无菌小鼠动物模型极为相似。因此采用抗生素处理建立伪无菌小鼠动物模型可用来预示无菌小鼠试验的结果,是研究肠道菌群与某些疾病的理想动物模型。
英文摘要:
      Gut microbiotas are closely related with the obesity of their host. Pseudo germ-free mice are a useful animal model for studying the relationship of gut microbiota with obesity and other related diseases. Twenty C57BL/6J eight-week-old male mice were administered with antibiotic cocktails in drinking water to remove the gut microbiota to establish the pseudo germ-free mouse model. These mice were randomly divided into two groups: control (CONV) group and high fat (HF) group, which were fed with a normal diet and a high-fat diet for eight weeks, respectively. Meanwhile, 20 specific-pathogen-free (SPF) mice were used as controls. Body weight, serum glucose levels, serum lipid levels, and insulin concentrations of the mice were measured by normal methods, and the areas of fat cells were calculated using software. The results revealed that body weight, fat pad, and perirenal fat weight of the HF pseudo germ-free mouse group were significantly lower than those of the HF SPF mouse group (p<0.001), and the insulin level and areas of fat cells in the HF pseudo germ-free mouse group were also significantly lower than those of SPF mice(p<0.01). Pseudo germ-free mice in the HF diet group showed low bodyweight and a low-fat phenotype, which were extremely similar to those of germ-free mice. As a result, the pseudo germ-free mouse model, established using antibiotic cocktails, can be used to predict modulation results in germ-free mice and is an ideal animal model for exploring the relationship between gut microbiota and diseases.
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