[关键词]
[摘要]
本文选用人宫颈鳞癌细胞SiHa进行体外抗肿瘤活性试验,并通过划痕实验对其抗肿瘤活性进行验证;同时选用昆明小鼠脾脏细胞的体外增殖试验和小鼠腹腔巨噬细胞RAW264.7吞噬中性红以及刺激巨噬细胞产生NO和IL-6的试验,探讨正红菇子实体多糖(HAP-I)的抗癌和免调节疫活性。结果显示,正红菇多糖在125~2000 μg/mL的范围内对SiHa细胞的增殖抑制作用随着浓度的增大而增大,并有一定的剂量依赖性;通过癌细胞划痕实验,证实其对癌细胞SiHa增殖有抑制作用。多糖浓度在10~500 μg/mL范围内能够促进淋巴细胞的增殖,且能协同LPS和ConA显著促进淋巴细胞的增殖,HAP-I协同LPS在50 μg/mL和100 μg/mL浓度时增殖作用显著(p<0.05),HAP-I协同ConA在100 μg/mL浓度时协同效果最佳。HAP-I能增强巨噬细胞吞噬中性红的能力,并且促进巨噬细胞分泌NO和IL-6,表明正红菇多糖有较好的免疫活性。
[Key word]
[Abstract]
Human cervical squamous cell carcinoma (SiHa) cells were chosen to study the in vitro antitumor activities of a Russula vinosa polysaccharide (HAP-I), and the results were confirmed using the scratch assay. Furthermore, an in vitro proliferation test with spleen cells from Kunming mice, neutral red uptake experiments, and analyses of the secretion of nitric oxide (NO) and interleukin-6 (IL-6) by RAW264.7 macrophages were conducted to study the anticancer and immunomodulatory activities of HAP-I. The results showed that in a concentration range of 125~2000 μg/mL, the inhibitory effect of HAP-I on SiHa was increased with increasing concentration and was dose-dependent to some extent. The scratch assay also demonstrated the inhibitory effect of HAP-I on the proliferation of SiHa. HAP-I could promote the proliferation of spleen cells from Kunming mice over a concentration range of 10~500 μg/mL, and this proliferation was dramatically promoted by the synergistic effect of concanavalin A (ConA) and lipopolysaccharide (LPS). The combination of HAP-I (at a concentration of 50 μg/mL or 100 μg/mL) and LPS had a significant effect on the proliferation; the optimal synergistic effect of HAP-I with ConA was found at 100 μg/mL. HAP-1 not only enhanced the ability of macrophages for neutral red uptake, but also increased the secretion of NO and IL-6 by macrophages, indicating that HAP-I has strong immunological activities.
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[基金项目]
广东省自然科学基金项目(2014A030313242)