研究了土茯苓总黄酮及主要成分落新妇苷对高脂小鼠脂肪代谢及体内抗氧化水平的影响。80只ICR小鼠随机分成8组，其中Ⅰ组为基础对照组，Ⅱ组为高脂对照组；其余6组在饮用水中分别添加1、2和4 mg/mL的落新妇苷或土茯苓总黄酮。八周后，解剖小鼠，采集腹腔脂肪和肝脏并称重，分析血清中甘油三酯(TG)，总胆固醇，高密度胆固醇和葡萄糖含量。实验结果表明与高脂对照组相比，土茯苓黄酮对小鼠摄食量和饮水量无明显影响，但可显著减少小鼠体重增幅、腹腔脂肪重量及血清中TG含量。机制研究表明土茯苓黄酮能显著提升小鼠肝脏中肉碱脂酰基转移酶和脂肪酸β-氧化酶活性，但对脂肪酸合成酶活性无明显影响，说明土茯苓黄酮通过促进分解减少体内脂肪蓄积。土茯苓黄酮能显著增加肝脏中抗氧化酶活性，并减少脂质过氧化产物丙二醛的含量，说明土茯苓黄酮具有体内抗氧化活性。

The effects of total flavonoids from Rhizoma Smilacis glabrae (RSG) and its main ingredient–astilbin–on the lipid metabolism and antioxidation level in mice were investigated. Eighty ICR mice were randomly assigned to eight groups. Group I was a control group fed with a normal diet, and group II was a control group fed with a high-fat diet. T otal flavonoids and astilbin from RSG at concentrations of 1, 2, and 4 mg/mL were added to the drinking water for the other six groups, respectively. After eight weeks, the mice were dissected, the intraperitoneal adipose tissue (IPAT) and liver were collected and weighed, and the serum triglyceride (TG), total cholesterol, high-density cholesterol, and glucose levels were determined. The results showed that compared with the high-fat control group, RSG flavonoids had no effect on the food and water intake in mice, but could significantly reduce body weight gain and IPAT weight, as well as the TG content in serum. The mechanism study indicated that RSG flavonoids significantly improved the activities of carnitine acyltransferase and fatty acid beta-oxidase in mouse liver, but had no effect on the activity of fatty acid synthase, indicating that RSG flavonoids may reduce fat accumulation by promoting fat decomposition. RSG flavonoids could significantly enhance the activity of antioxidant enzymes and decrease the level of malondialdehyde (MDA), indicating that RSG flavonoids have in vivo antioxidant activity.

江西省自然科学基金资助项目(20122BAB214005)；江西省天然产物与功能食品重点实验室开放基金项目(2015003)