本文研究了萝卜籽油的组成及其对小鼠的通便作用。通过化学方法测定了萝卜籽油的各项理化指标、脂肪酸组成及多种活性物质含量；以高、中、低三个浓度的萝卜籽油喂食小鼠及便秘模型小鼠，检测小鼠排便重量、粒数、首粒排黑便时间、墨汁推进率。结果表明：萝卜籽油含多种脂肪酸，其中芥酸含量为36.18%，亚麻酸20.22%、油酸19.80%、亚油酸12.35%，其余脂肪酸含量很少；油脂中的不饱和脂肪酸占总脂肪酸的88.74%。萝卜籽油中含有维生素E 1.5984 mg/g、莱菔素55.35 mg/kg、甾醇14.3201 mg/g。用25%~100%的萝卜籽油取代基础饲料中的大豆油，有明显促进小鼠排便的作用。灌胃1.0 g~3.0 g/(kg?bw)萝卜籽油，具有明显改善便秘小鼠通便及推动便秘小鼠小肠蠕动的作用。2.0 g~3.0 g/(kg?bw)萝卜籽油几乎可以达到治愈小鼠便秘的目的。萝卜籽油品质优良，具有通便作用，开发前景广阔。

The composition of radish seed oil and its laxative effects in mice were investigated. The physicochemical indices, fatty acid composition, and the content of various active substances were measured using chemical methods. High, medium, and low dosages of radish seed oil were administered to mice by gavage, and the fecal mass, number of fecal pellets, time of the first black pellet, and ink propulsion rate were determined. Several types of fatty acids were identified in radish seed oil, including 36.18% erucic acid, 20.22% linolenic acid, 19.80% oleic acid, 12.35% linoleic acid, and small amounts of other fatty acids. Unsaturated fatty acids accounted for 88.74% of the total fatty acids. The content of tocopherol, sulforaphene, and sterol in radish seed oil was 1.5984 mg/g, 55.35 mg/kg, and 14.3201 mg/g, respectively. Replacing the soybean oil in basal feed formula with 25~100% radish seed oil showed a significant anti-constipation effect in mice. Mice gavage-fed with radish seed oil at 1.0~3.0 g/(kg?bw) showed significantly improved small intestine peristalsis when constipated. Constipated mice were almost cured by radish seed oil doses of 2.0~3.0 g/(kg?bw). Radish seed oil has several beneficial qualities, including anti-constipation effects, and shows a broad application prospect.

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